Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide

被引:115
作者
Qin, Tao [1 ]
Chen, Jin [2 ]
Wang, Deyun [1 ]
Hu, Yuanliang [1 ]
Zhang, Jing [1 ]
Wang, Mi [1 ]
Qiu, Shulei [1 ]
Gao, Zhenzhen [1 ]
Liu, Rongrong [1 ]
Yu, Yun [1 ]
Huang, Yee [1 ]
Wang, Qiaochu [3 ]
Wang, Qiaoxiu [1 ]
机构
[1] Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
[2] Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Peoples R China
[3] Nanjing Med Univ, Sch Basic Med Sci, Dept Biotechnol, Nanjing 210029, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Selenizing Chinese angelica polysaccharide (sCAP); Lymphocyte proliferation; Antibody titer; IFN-gamma; IL-6; HERBAL MEDICINAL INGREDIENTS; SULFATED POLYSACCHARIDES; CHICKEN; VACCINATION; PROFILES; DISEASE;
D O I
10.1016/j.carbpol.2013.02.072
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
On the basis of previous test that selenizing Chinese angelica polysaccharides (sCAPs) with stronger immune-enhancing activity in vitro were picked out, the immune-enhancing activity in vivo of three sCAPs, sCAP(2), sCAP(6) and sCAP(8), at high and low dosage were compared taking the unmodified Chinese angelica polysaccharide (CAP) as control by determination of peripheral lymphocyte proliferation, serum antibody titer, IFN-gamma and IL-6 contents in chicken vaccinated with Newcastle Disease vaccine. The results showed that three sCAPs at suitable dosage could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-gamma and IL-6 contents as compared with unmodified CAP, sCAP(2) at low dosage possessed the strongest action. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP(2) possessed the best efficacy and would be as a component drug of new-type immunoenhancer. (c) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:183 / 187
页数:5
相关论文
共 30 条
[1]
Screening on the immune-enhancing active site of Siberian solomonseal rhizome polysaccharide [J].
Abula, Saifuding ;
Wang, Junmin ;
Hu, Yuanliang ;
Wang, Deyun ;
Sheng, Xin ;
Zhang, Jing ;
Zhao, Xiaona ;
The Luong Nguyen ;
Zhang, Yuqing .
CARBOHYDRATE POLYMERS, 2011, 85 (03) :687-691
[2]
Cao J., 2010, CHINESE FOLK MED, V19, P61
[3]
Effect of (1→3)- and (1→4)-linkages of fully sulfated polysaccharides on their anticoagulant activity [J].
Chaidedgumjorn, A ;
Toyoda, H ;
Woo, ER ;
Lee, KB ;
Kim, YS ;
Toida, T ;
Imanari, T .
CARBOHYDRATE RESEARCH, 2002, 337 (10) :925-933
[4]
CHIHARA G, 1969, Nature (London), V222, P687
[5]
Cui Q, 2003, CHINESE J BIOCH PHAR, V24, P155
[6]
Hyaluronan: polysaccharide chaos to protein organisation [J].
Day, AJ ;
Sheehan, JK .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2001, 11 (05) :617-622
[7]
Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions [J].
Fukui, S ;
Feizi, T ;
Galustian, C ;
Lawson, AM ;
Chai, WG .
NATURE BIOTECHNOLOGY, 2002, 20 (10) :1011-1017
[8]
[高建忠 GAO Jianzhong], 2006, [分析科学学报, Journal of Analytical Science], V22, P157
[9]
Grobe K., 2001, BIOCH BIOPHYSICS ACT, V1573, P209
[10]
[季敬璋 Ji Jingzhang], 2003, [中国免疫学杂志, Chinese Journal of Immunology], V19, P611