Transfected synphilin-1 forms cytoplasmic inclusions in HEK293 cells

被引：55

作者：

O'Farrell, C

Murphy, DD

Petrucelli, L

Singleton, AB

Hussey, J

Farrer, M

Hardy, J

Dickson, DW

Cookson, MR

机构：

[1] Mayo Clin Jacksonville, Dept Neurosci, Neurogenet Lab, Jacksonville, FL 32224 USA

[2] Mayo Clin Jacksonville, Neuropathol Lab, Jacksonville, FL 32224 USA

[3] NINCDS, Ctr Neurosci, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR BRAIN RESEARCH | 2001年 / 97卷 / 01期

关键词：

Parkinson's disease; Green fluorescent protein; protein-protein interaction; synuclein;

D O I：

10.1016/S0169-328X(01)00292-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The discovery of mutations in the gene for alpha -synuclein in familial Parkinson's disease (PD) has led to an increased interest in this pre-synaptic protein. Synphilin-1, a potential synuclein-binding protein, was cloned using yeast two-hybrid assays. The function of synphilin-1 is currently unknown, although it has been reported to be present along with alpha -synuclein in Lewy bodies in PD. In the present study, we monitored synphilin-1 aggregation directly using fusion proteins of synphilin-1 and green fluorescent protein (EGFP). Transfection of synphilin-EGFP fusion proteins formed cytoplasmic inclusions in HEK293 cells. Although these inclusions overlapped with the distribution of alpha -synuclein, they were unlike Lewy bodies in that they were not eosinophilic, and instead were membrane-bound, lipid-rich cytoplasmic inclusions. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：94 / 102

页数：9

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