Mechanotransduction in the lung -: Cyclic stretch activates ERK1/2 via G proteins and EGFR in alveolar epithelial cells

被引:97
作者
Correa-Meyer, E
Pesce, L
Guerrero, C
Sznajder, JI
机构
[1] Northwestern Univ, Div Pulm & Crit Care Med, Chicago, IL 60611 USA
[2] Univ Salamanca, Ctr Invest Canc, Salamanca 37007, Spain
关键词
mechanotransduction; mechanical stress; mitogen-activated protein kinase; lung injury; epidermal growth factor receptor;
D O I
10.1152/ajplung.00203.2001
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Mechanical stimuli are transduced into intracellular signals in lung alveolar epithelial cells (AEC). We studied whether mitogen-activated protein kinase (MAPK) pathways are activated during cyclic stretch of AEC. Cyclic stretch induced a rapid (within 5 min) increase in extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in AEC. The inhibition of Na+, L-type Ca2+ and stretch-activated ion channels with amiloride, nifedipine, and gadolinium did not prevent the stretch-induced ERK1/2 activation. The inhibition of Grb2-SOS interaction with an SH3 binding sequence peptide, Ras with a farnesyl transferase inhibitor, and Raf-1 with forskolin did not affect the stretch-induced ERK1/2 phosphorylation. Moreover, cyclic stretch did not increase Ras activity, suggesting that stretch-induced ERK1/2 activation is independent of the classical receptor tyrosine kinase-MAPK pathway. Pertussis toxin and two specific epidermal growth factor receptor (EGFR) inhibitors (AG-1478 and PD-153035) prevented the stretch-induced ERK1/2 activation. Accordingly, in primary AEC, cyclic stretch activates ERK1/2 via G proteins and EGFR, in Na+ and Ca2+ influxes and Grb2-SOS-, Ras-, and Raf-1-independent pathways.
引用
收藏
页码:L883 / L891
页数:9
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