The expression of TRAIL and its receptors in gastric cancer and the apoptotic effect of rh-TRAIL on SGC7901 cells

被引：18

作者：

Hu, Jian-Kun ^{[1
]}

Yang, Kun ^{[1
]}

Li, Chun-Mei ^{[2
]}

Zhang, Bo ^{[1
]}

Chen, Zhi-Xin ^{[1
]}

Chen, Xin-Zu ^{[1
]}

Chen, Jia-Ping ^{[1
]}

机构：

[1] Sichuan Univ, W China Hosp, Dept Surg Gastroenterol, Chengdu 610041, Sichuan Prov, Peoples R China

[2] Sichuan Univ, W China Hosp 2, Dept Gynecol & Obstet, Chengdu 610041, Sichuan Prov, Peoples R China

来源：

ONCOLOGY REPORTS | 2009年 / 21卷 / 03期

关键词：

TNF-related apoptosis-inducing ligand; gastric cancer; apoptosis; chemotherapeutic drugs; CYTOTOXIC LIGAND TRAIL; CHEMOTHERAPEUTIC DRUGS; MEDIATED APOPTOSIS; ANTITUMOR-ACTIVITY; OVARIAN-CARCINOMA; DECOY RECEPTOR; UP-REGULATION; IN-VITRO; TUMOR; DEATH;

D O I：

10.3892/or_00000271

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study investigated the expression of TRAIL and its receptors in human gastric cancer and normal gastric tissues, the effects of rh-TRAIL with or without chemotherapeutic drugs in apoptosis of the gastric cancer cell line SGC7901 and the expression changes of DR4 and DR5 in SGC7901 cells influenced by chemotherapeutic drugs. The expression of TRAIL, DR4 and DcR1 were studied in 34 cases of human gastric cancer tissues and 15 cases of adjacent normal mucosa tissues, as well as 21 cases of distant normal mucosa tissues by means of immunohistochemistry. In addition, the expression of FasL were studied in gastric cancer tissues by immunohistochemistry. The effects of treatment with rh-TRAIL alone and/or chemotherapeutic drugs on SGC7901 cell growth inhibition were measured by MTT assay and the mRNA changes of DR4 and DR5 were detected by RT-PCR technique. The expression of TRAIL, DR4 and DeR1 in gastric cancer were lower than those of normal tissues (P<0.05). There was significant relationship between the expression of TRAIL and Borrmann type of gastric cancer (P=0.039), and so was the expression of DcR1 and tumor location (P=0.01). The correlation coefficient between the expression of TRAIL and FasL was 0.354 (P=0.04). Rh-TRAIL protein had inhibiting effect on the growth of SGC7901 cells. DDP and 5-FU increased the growth-inhibiting ability of rh-TRAIL to SGC7901 cells. DDP facilitated the induction of expression of DR4 and DR5 significantly in cell line (P<0.05), but 5-FU influenced only the expression of DR5 significantly. From the results, we concluded that the expression of TRAIL and its receptors were lower in gastric cancer than those of normal tissue, and the apoptosis-inducing effect of rh-TRAIL was enhanced when concomitant with chemotherapeutic drugs.

引用

页码：681 / 688

页数：8

共 44 条

[1] Overexpression of Bcl2 abrogates chemo- and radiotherapy-induced sensitisation of NCI-H460 non-small-cell lung cancer cells to adenovirus-mediated expression of full-length TRAIL [J].

Abou el Hassan, MAI ;

Mastenbroek, DCJ ;

Gerritsen, WR ;

Giaccone, G ;

Kruyt, FAE .

BRITISH JOURNAL OF CANCER, 2004, 91 (01) :171-177

[2]

[Anonymous], NCCN CLIN PRACT GUID

[3] Chemotherapy induces death receptor 5 in epithelial ovarian carcinoma [J].

Arts, HJG ;

de Jong, S ;

Hollema, H ;

ten Hoor, K ;

van der Zee, AGJ ;

de Vries, EGE .

GYNECOLOGIC ONCOLOGY, 2004, 92 (03) :794-800

[4] Safety and antitumor activity of recombinant soluble Apo2 ligand [J].

Ashkenazi, A ;

Pai, RC ;

Fong, S ;

Leung, S ;

Lawrence, DA ;

Masters, SA ;

Blackie, C ;

Chang, L ;

McMurtrey, AE ;

Hebert, A ;

DeForge, L ;

Koumenis, IL ;

Lewis, D ;

Harris, L ;

Bussiere, J ;

Koeppen, H ;

Shahrokh, Z ;

Schwall, RH .

JOURNAL OF CLINICAL INVESTIGATION, 1999, 104 (02) :155-162

[5] Ligand-based targeting of apoptosis in cancer: The potential of recombinant human apoptosis ligand 2/tumor necrosis factor-related apoptosis-inducing ligand (rhApo2L/TRAIL) [J].

Ashkenazi, Avi ;

Holland, Pamela ;

Eckhardt, S. Gail .

JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (21) :3621-3630

[6] Chemotherapeutic drugs sensitize cancer cells to TRAIL-mediated apoptosis: up-regulation of DR5 and inhibition of Yin Yang 1 [J].

Baritaki, Stavroula ;

Huerta-Yepez, Sara ;

Sakai, Toshiyuki ;

Spandidos, Demetrios A. ;

Bonavida, Benjamin .

MOLECULAR CANCER THERAPEUTICS, 2007, 6 (04) :1387-1399

[7] GM1 and tumor necrosis factor-α, overexpressed in rena cell carcinoma, synergize to induce T-cell apoptosis [J].

Das, Tanya ;

Sa, Gaurisankar ;

Hilston, Cynthia ;

Kudo, Daisuke ;

Rayman, Patricia ;

Biswas, Kaushik ;

Molto, Luis ;

Bukowski, Ronald ;

Rini, Brian ;

Finke, James H. ;

Tannenbaum, Charles .

CANCER RESEARCH, 2008, 68 (06) :2014-2023

[8] Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma [J].

Dong, Hiep Phuc ;

Kleinberg, Lilach ;

Silins, Ilvars ;

Florenes, Vivi Ann ;

Trope, Claes G. ;

Risberg, Bjoern ;

Nesland, Jahn M. ;

Davidson, Ben .

CANCER, 2008, 112 (01) :84-93

[9] Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL [J].

Emery, JG ;

McDonnell, P ;

Burke, MB ;

Deen, KC ;

Lyn, S ;

Silverman, C ;

Dul, E ;

Appelbaum, ER ;

Eichman, C ;

DiPrinzio, R ;

Dodds, RA ;

James, IE ;

Rosenberg, M ;

Lee, JC ;

Young, PR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (23) :14363-14367

[10]

FERNANDES BJ, 1991, CAN J SURG, V34, P349

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