Detection and localization of single molecular recognition events using atomic force microscopy

被引:782
作者
Hinterdorfer, P
Dufrêne, YF
机构
[1] Johannes Kepler Univ, Inst Biophys, A-4040 Linz, Austria
[2] Univ Catholique Louvain, Lab Interfaces Chem, B-1348 Louvain, Belgium
关键词
D O I
10.1038/NMETH871
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Because of its piconewton force sensitivity and nanometer positional accuracy, the atomic force microscope (AFM) has emerged as a powerful tool for exploring the forces and the dynamics of the interaction between individual Ligands and receptors, either on isolated molecules or on cellular surfaces. These studies require attaching specific biomolecules or cells on AFM tips and on solid supports and measuring the unbinding forces between the modified surfaces using AFM force spectroscopy. In this review, we describe the current methodology for molecular recognition studies using the AFM, wit h an emphasis on strategies available for preparing AFM tips and samples, and on procedures for detecting and localizing single molecular recognition events.
引用
收藏
页码:347 / 355
页数:9
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