Microdetermination of proteins by Rayleigh light scattering technique with acid green 25

被引：75

作者：

Ma, CQ ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] BEIJING UNIV,DEPT CHEM,BEIJING 100871,PEOPLES R CHINA

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 338卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Rayleigh light scattering; proteins; acid green 25;

D O I：

10.1016/S0003-2670(96)00444-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on the enhancement of Rayleigh light scattering (RLS) of acid green 25 (Ag25) by proteins, a new quantitative determination method for proteins in aqueous solutions has been developed. This assay is very sensitive (0.136-10.20 mu g/ml), rapid (<2 min) and simple (one-step assay and common fluorimeter). Differences between the various proteins can be related to their isoelectric points. There is little or no interference from amino acids, most of the metal ions or complexing agents (e.g. EDTA). Interferences by some metal ions, detergents and salts can be minimized by dilution.

引用

页码：255 / 260

页数：6

共 14 条

[1] DEPOLARIZED RAYLEIGH LIGHT-SCATTERING IN ABSORPTION-BANDS MEASURED IN LYCOPENE SOLUTION [J].