Production of trehalose from starch by thermostable enzymes from Sulfolobus acidocaldarius

被引：46

作者：

Mukai, K ^{[1
]}

Tabuchi, A ^{[1
]}

Nakada, T ^{[1
]}

Shibuya, T ^{[1
]}

Chaen, H ^{[1
]}

Fukuda, S ^{[1
]}

Kurimoto, M ^{[1
]}

Tsujisaka, Y ^{[1
]}

机构：

[1] HAYASHIBARA INST CORP, OKAYAMA 700, JAPAN

来源：

STARCH-STARKE | 1997年 / 49卷 / 01期

关键词：

D O I：

10.1002/star.19970490107

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The optimum conditions for the production of trehalose from starch were investigated using two thermostable enzymes, maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase), from Sulfolobus acidocaldarius ATCC 33909. The optimum pH was 5.5 and the optimum temperature was 55-57 degrees C using isoamylase from Pseudomonas amyloderamosa as a debranching enzyme. The addition of CGTase to the reaction mixture during the saccharification process caused an increase in trehalose and a decrease in maltose and maltotriose. Isoamylase was better than pullulanase as a debranching enzyme. The yield of trehalose was independent of the type of starch used. Under optimum conditions, the yield of trehalose from corn starch at 30% concentration was more than 82%.

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页码：26 / 30

页数：5

相关论文

共 21 条

[1]

BENDER H, 1961, BIOCHEM Z, V334, P79

[2] QUALITATIVE TEST FOR CARBOHYDRATE MATERIAL [J].

DREYWOOD, R .

INDUSTRIAL AND ENGINEERING CHEMISTRY-ANALYTICAL EDITION, 1946, 18 (08) :499-499

[3]

Elbein A D, 1974, Adv Carbohydr Chem Biochem, V30, P227, DOI 10.1016/S0065-2318(08)60266-8

[4]

LAMA L, 1991, BIOTECH FORUM EUROPE, V8, P201

[5] FORMATION OF TREHALOSE FROM MALTOOLIGOSACCHARIDES BY A NOVEL ENZYMATIC SYSTEM [J].

MARUTA, K ;

NAKADA, T ;

KUBOTA, M ;

CHAEN, H ;

SUGIMOTO, T ;

KURIMOTO, M ;

TSUJISAKA, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1995, 59 (10) :1829-1834

[6]

Myrback K, 1936, BIOCHEM Z, V288, P329

[7] Purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from Arthrobacter sp Q36 [J].

Nakada, T ;

Maruta, K ;

Mitsuzumi, H ;

Kubota, M ;

Chaen, H ;

Sugimoto, T ;

Kurimoto, M ;

Tsujisaka, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1995, 59 (12) :2215-2218

[8] Purification and characterization of thermostable maltooligosyl trehalose trehalohydrolase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius [J].

Nakada, T ;

Ikegami, S ;

Chaen, H ;

Kubota, M ;

Fukuda, S ;

Sugimoto, T ;

Kurimoto, M ;

Tsujisaka, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (02) :267-270

[9] Purification and characterization of thermostable maltooligosyl trehalose synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius [J].

Nakada, T ;

Ikegami, S ;

Chaen, H ;

Kubota, M ;

Fukuda, S ;

Sugimoto, T ;

Kurimoto, M ;

Tsujisaka, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (02) :263-266

[10] Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36 [J].

Nakada, T ;

Maruta, K ;

Tsusaki, K ;

Kubota, M ;

Chaen, H ;

Sugimoto, T ;

Kurimoto, M ;

Tsujisaka, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1995, 59 (12) :2210-2214