AMPK regulation of mouse oocyte meiotic resumption in vitro

被引:81
作者
Chen, J
Hudson, E
Chi, MM
Chang, AS
Moley, KH
Hardie, DG
Downs, SM [1 ]
机构
[1] Marquette Univ, Dept Biol Sci, Milwaukee, WI 53201 USA
[2] Univ Dundee, Div Mol Physiol, Dundee, Scotland
[3] Washington Univ, Sch Med, Dept Obstet & Gynecol, St Louis, MO 63110 USA
基金
英国惠康基金;
关键词
mouse oocytes; AMP-activated protein kinase; meiotic resumption; compound C;
D O I
10.1016/j.ydbio.2005.11.039
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have previously shown that the adenosine analog 5-aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR), an activator of AMP-activated protein kinase (AMPK), stimulates an increase in AMPK activity and induces meiotic resumption in mouse oocytes [Downs, S.M., Hudson, E.R., Hardie, D.G., 2002. A potential role for AMP-activated protein kinase in meiotic induction in mouse oocytes. Dev. Biol, 245, 200-212]. The present study was carried out to better define a causative role for AMPK in oocyte meiotic maturation. When microinjected with a constitutively active AMPK, about 20% of mouse oocytes maintained in meiotic arrest with dibutyryl cAMP (dbcAMP) were stimulated to undergo germinal vesicle breakdown (GVB), while there was no effect of catalytically dead kinase. Western blot analysis revealed that germinal vesicle (GV)-stage oocytes cultured in dbcAMP-containing medium plus AICAR possessed elevated levels of active AMPK, and this was confirmed by AMPK assays using a peptide substrate of AMPK to directly measure AMPK activity. AICAR-induced meiotic resumption and AMPK activation were blocked by compound C or adenine 9-beta-D-arabinofuranoside (araA, a precursor of araATP), both inhibitors of AMPK. Compound C failed to suppress adenosine uptake and phosphorylation, indicating that it did not block AICAR action by preventing its metabolism to the AMP analog, ZMP. 2'-Deoxycoformycin (DCF), a potent adenosine deaminase inhibitor, reversed the inhibitory effect of adenosine on oocyte maturation by modulating intracellular AMP levels and activating AMPK. Rosiglitazone, an antidiabetic agent, stimulated AMPK activation in oocytes and triggered meiotic resumption. In spontaneously maturing oocytes, GVB was preceded by AMPK activation and blocked by compound C. Collectively, these results support the proposition that active AMPK within mouse oocytes provides a potent meiosis-inducing signal in vitro. (C) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:227 / 238
页数:12
相关论文
共 47 条
  • [1] ALEXANDRE H, 1991, DEVELOPMENT, V112, P971
  • [2] INVOLVEMENT OF CAMP-DEPENDENT PROTEIN-KINASE AND PROTEIN-PHOSPHORYLATION IN REGULATION OF MOUSE OOCYTE MATURATION
    BORNSLAEGER, EA
    MATTEI, P
    SCHULTZ, RM
    [J]. DEVELOPMENTAL BIOLOGY, 1986, 114 (02) : 453 - 462
  • [3] Thiazolidinediones, like metformin, inhibit respiratory complex I -: A common mechanism contributing to their antidiabetic actions?
    Brunmair, B
    Staniek, K
    Gras, F
    Scharf, N
    Althaym, A
    Clara, R
    Roden, M
    Gnaiger, E
    Nohl, H
    Waldhäusl, W
    Fürnsinn, C
    [J]. DIABETES, 2004, 53 (04) : 1052 - 1059
  • [4] Maternal hyperglycemia adversely affects preovulatory oocyte metabolism.
    Chang, AS
    Chi, MM
    Moley, KH
    [J]. FERTILITY AND STERILITY, 2004, 82 : S269 - S269
  • [5] Metabolic changes in the glucose-induced apoptotic blastocyst suggest alterations in mitochondrial physiology
    Chi, MMY
    Hoehn, A
    Moley, KH
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2002, 283 (02): : E226 - E232
  • [6] Role of cyclic nucleotide signaling in oocyte maturation
    Conti, M
    Andersen, CB
    Richard, F
    Mehats, C
    Chun, SY
    Horner, K
    Jin, C
    Tsafriri, A
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2002, 187 (1-2) : 153 - 159
  • [7] CORTON JM, 1995, EUR J BIOCHEM, V229, P558, DOI 10.1111/j.1432-1033.1995.tb20498.x
  • [8] DIURNAL RHYTHM OF PHOSPHORYLATION OF RAT-LIVER ACETYL COA CARBOXYLASE BY THE AMP-ACTIVATED PROTEIN-KINASE, DEMONSTRATED USING FREEZE-CLAMPING - EFFECTS OF HIGH-FAT DIETS
    DAVIES, SP
    CARLING, D
    MUNDAY, MR
    HARDIE, DG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (03): : 615 - 623
  • [9] TISSUE DISTRIBUTION OF THE AMP-ACTIVATED PROTEIN-KINASE, AND LACK OF ACTIVATION BY CYCLIC-AMP-DEPENDENT PROTEIN-KINASE, STUDIED USING A SPECIFIC AND SENSITIVE PEPTIDE ASSAY
    DAVIES, SP
    CARLING, D
    HARDIE, DG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2): : 123 - 128
  • [10] LOCATION AND FUNCTION OF 3 SITES PHOSPHORYLATED ON RAT ACETYL-COA CARBOXYLASE BY THE AMP-ACTIVATED PROTEIN-KINASE
    DAVIES, SP
    SIM, ATR
    HARDIE, DG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (01): : 183 - 190