Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials

被引：8

作者：

Dooms, M. ^{[1
]}

Chango, A. ^{[1
]}

Barbour, E. ^{[2
]}

Pouillart, P. ^{[1
]}

Nour, A. M. Abdel ^{[1
]}

机构：

[1] LaSalle Beauvais, F-60000 Beauvais, France

[2] Amer Univ Beirut, Dept Anim & Vet Sci, Beirut 11072020, Lebanon

来源：

METHODS | 2013年 / 59卷 / 01期

关键词：

Clinical trial; MIQE qPCR; Transcriptional biomarkers;

D O I：

10.1016/j.ymeth.2012.07.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The "Minimum Information for the Publication of qPCR Experiments" (MIQE [3]) guidelines are very much targeted at basic research experiments and have to our knowledge not been applied to qPCR assays carried out in the context of clinical trials. This report details the use of the MIQE qPCR app for iPhone (App Store, Apple) to assess the MIQE compliance of one clinical and five pre-clinical trials. This resulted in the need to include 14 modifications that make the guidelines more relevant for the assessment of this special type of application. We also discuss the need for flexibility, since while some parameters increase experimental quality, they also require more reagents and more time, which is not always feasible in a clinical setting. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.

引用

页码：147 / 153

页数：7

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