Profiling of differential protein expression in angiogenin-induced HUVECs using antibody-arrayed ProteoChip

被引：28

作者：

Ahn, EH

Kang, DK

Chang, SI

Kang, CS

Han, MH

Kang, IC ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Biotechnol Res Inst, Prot Chip Res Ctr, Cheongju 361763, South Korea

[2] Chungbuk Natl Univ, Dept Biochem, Cheongju 361763, South Korea

[3] Hoseo Univ, Dept Biol Sci, Asan, South Korea

[4] Proteogen Inc, Seoul, South Korea

来源：

PROTEOMICS | 2006年 / 6卷 / 04期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

angiogenin; antibody microarray; chip-based proteomics; ProteoChip; protein expression profiling;

D O I：

10.1002/pmic.200500394

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

ProteoChip has been developed as a novel protein microarray technology. So far it has been applied in new lead screening and molecular diagnostics and we expect its role to grow in the field of biology. Here, we investigated the application of ProteoChip for the study of differential protein expression profiles in angiogenin-induced human umbilical vein endothelial cells (HUVECs). Antibody microarrays constructed by immobilizing 60 distinct antibodies against signal-transducing proteins on ProteoChip base plates were used to analyze the expression pattern of cell-signaling proteins in HUVECs treated with angiogenin. The antibody microarray approach showed that angiogenin induced the up- and down-regulation of several cellular regulators related with cell proliferation. Changes in the expression of signaling proteins determined by antibody microarray were validated by Western blot analysis. In this experiment, ten up-regulated proteins and six down-regulated proteins were identified and confirmed by immunoblot analysis. Taken together, these data suggest that antibody microarrays using ProteoChip technology can be a powerful tool for high-throughput analysis of proteomes in biological samples.

引用

页码：1104 / 1109

页数：6

共 32 条

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