Use of adoptive transfer of T-cell-antigen-receptor-transgenic T cells for the study of T-cell activation in vivo

被引：174

作者：

Pape, KA ^{[1
]}

Kearney, ER ^{[1
]}

Khoruts, A ^{[1
]}

Mondino, A ^{[1
]}

Merica, R ^{[1
]}

Chen, ZM ^{[1
]}

Ingulli, E ^{[1
]}

White, J ^{[1
]}

Johnson, JG ^{[1
]}

Jenkins, MK ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT MICROBIOL,MINNEAPOLIS,MN 55455

来源：

IMMUNOLOGICAL REVIEWS | 1997年 / 156卷

关键词：

D O I：

10.1111/j.1600-065X.1997.tb00959.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Adoptive transfer of TCR-transgenic T cells uniformly expressing an identifiable TCR of known peptide/MHC specificity can be used to monitor the in vivo behavior of antigen-specific T cells. We have used this system to show that naive T cells are initially activated within the T-cell zones of secondary lymphoid tissue to proliferate in a B7-dependent manner. If adjuvants or inflammatory cytokines are present during this period, enhanced numbers of T cells accumulate, migrate into B-cell-rich follicles, and acquire the capacity to produce IFN-gamma and help B cells produce IgG2a. If inflammation is absent, most of the initially activated antigen-specific T cells disappear without entering the follicles, and the survivors are poor producers of IL-2 and IFN-gamma. Our results indicate that inflammatory mediators play a key role in regulating the anatomic location, clonal expansion, survival and lymphokine production potential of antigen-stimulated T cells in vivo.

引用

页码：67 / 78

页数：12

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[1] CD40 ligand is required for protective cell-mediated immunity to Leishmania major [J].