Mechanism-based anti-anxiety effects of polysaccharides extracted from Shudihuang (Radix Rehmanniae Preparata) by two-dimensional electrophoresis analysis in rat hippocampus proteins

被引：31

作者：

Cui, Ying ^{[1
]}

Rong, Chunlei ^{[2
]}

Wang, Junming ^{[1
,3
]}

Cui, Can ^{[4
]}

Wang, Li ^{[5
]}

Feng, Zhiyi ^{[1
]}

Feng, Jing ^{[1
]}

Niu, Bing ^{[6
]}

机构：

[1] Henan Univ Tradit Chinese Med, Sch Pharm, Zhengzhou 450046, Peoples R China

[2] Henan Univ Tradit Chinese Med, Dept Pharm, Affiliated Hosp 1, Zhengzhou 450000, Peoples R China

[3] Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China

[4] Xian Univ Technol, Dept Ideol & Polit Educ, Xian 710054, Peoples R China

[5] Zhengzhou Univ, Coll Basic Med, Zhengzhou 450001, Peoples R China

[6] Henan Prov Hosp Tradit Chinese Med, Dept Stomatol, Zhengzhou 450002, Peoples R China

来源：

JOURNAL OF TRADITIONAL CHINESE MEDICINE | 2013年 / 33卷 / 04期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

Electrophoresis; gel; two-dimensional; Beta-synuclein; DJ-1; protein; Peroxiredoxins; Dimethylargininase; Iron-sulfur proteins; Anti-anxiety agents;

D O I：

10.1016/S0254-6272(13)60159-4

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG). METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that beta-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of B-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats. (C) 2013 JTCM. All rights reserved.

引用

页码：524 / 530

页数：7

共 36 条

[1]

Pharmacological evidence for the role of nitric oxide in the modulation of stress-induced anxiety by morphine in rats [J].