Mechanism-based anti-anxiety effects of polysaccharides extracted from Shudihuang (Radix Rehmanniae Preparata) by two-dimensional electrophoresis analysis in rat hippocampus proteins

被引:31
作者
Cui, Ying [1 ]
Rong, Chunlei [2 ]
Wang, Junming [1 ,3 ]
Cui, Can [4 ]
Wang, Li [5 ]
Feng, Zhiyi [1 ]
Feng, Jing [1 ]
Niu, Bing [6 ]
机构
[1] Henan Univ Tradit Chinese Med, Sch Pharm, Zhengzhou 450046, Peoples R China
[2] Henan Univ Tradit Chinese Med, Dept Pharm, Affiliated Hosp 1, Zhengzhou 450000, Peoples R China
[3] Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China
[4] Xian Univ Technol, Dept Ideol & Polit Educ, Xian 710054, Peoples R China
[5] Zhengzhou Univ, Coll Basic Med, Zhengzhou 450001, Peoples R China
[6] Henan Prov Hosp Tradit Chinese Med, Dept Stomatol, Zhengzhou 450002, Peoples R China
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
Electrophoresis; gel; two-dimensional; Beta-synuclein; DJ-1; protein; Peroxiredoxins; Dimethylargininase; Iron-sulfur proteins; Anti-anxiety agents;
D O I
10.1016/S0254-6272(13)60159-4
中图分类号
R [医药、卫生];
学科分类号
100218 [急诊医学];
摘要
OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG). METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that beta-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of B-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats. (C) 2013 JTCM. All rights reserved.
引用
收藏
页码:524 / 530
页数:7
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