Effects of ceramide on liquid-ordered domains investigated by simultaneous AFM and FCS

被引：195

作者：

Chiantia, Salvatore ^{[1
]}

Kahya, Nicoletta ^{[1
]}

Ries, Jonas ^{[1
]}

Schwille, Petra ^{[1
]}

机构：

[1] Dresden Univ Technol, Biotechnol Zentrum, D-8027 Dresden, Germany

来源：

BIOPHYSICAL JOURNAL | 2006年 / 90卷 / 12期

关键词：

D O I：

10.1529/biophysj.106.081026

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The sphingolipid ceramides are known to influence lipid lateral organization in biological membranes. In particular, ceramide-induced alterations of microdomains can be involved in several cell functions, ranging from apoptosis to immune response. We used a combined approach of atomic force microscopy, fluorescence correlation spectroscopy, and confocal fluorescence imaging to investigate the effects of ceramides in model membranes of biological relevance. Our results show that physiological quantities of ceramide in sphingomyelin/dioleoylphosphatidylcholine/cholesterol supported bilayers lead to a significant rearrangement of lipid lateral organization. Our experimental setup allowed a simultaneous characterization of both structural and dynamic modi. cation of membrane microdomains, induced by the presence of ceramide. Formation of similar ceramide-enriched domains and, more general, alterations of lipid-lipid interactions can be of crucial importance for the biological function of cell membranes.

引用

页码：4500 / 4508

页数：9

共 36 条

[1] Sphingomyelin/cholesterol ratio: an important determinant of glucose transport mediated by GLUT-1 in 3T3-L1 preadipocytes [J].

Al-Makdissy, N ;

Younsi, M ;

Pierre, S ;

Ziegler, O ;

Donner, M .

CELLULAR SIGNALLING, 2003, 15 (11) :1019-1030

[2] Displacement of sterols from sterol/sphingomyelin domains in fluid bilayer membranes by competing molecules [J].

Alanko, SMK ;

Halling, KK ;

Maunula, S ;

Slotte, JP ;

Ramstedt, B .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1715 (02) :111-121

[3] The caveolae membrane system [J].

Anderson, RGW .

ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :199-225

[4] SPHINGOMYELINS IN BILAYERS AND BIOLOGICAL-MEMBRANES [J].

BARENHOLZ, Y ;

THOMPSON, TE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 604 (02) :129-158

[5] How to determine diffusion coefficients in planar phospholipid systems by confocal fluorescence correlation spectroscopy [J].

Benda, A ;

Benes, M ;

Marecek, V ;

Lhotsky, A ;

Hermens, WT ;

Hof, M .

LANGMUIR, 2003, 19 (10) :4120-4126

[6] Domain formation and stability in complex lipid bilayers as reported by cholestatrienol [J].

Björkqvist, YJE ;

Nyholm, TKM ;

Slotte, JP ;

Ramstedt, B .

BIOPHYSICAL JOURNAL, 2005, 88 (06) :4054-4063

[7] Ceramide-enriched membrane domains [J].

Bollinger, CR ;

Teichgräber, V ;

Gulbins, E .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2005, 1746 (03) :284-294

[8] Domain structure in model membrane bilayers investigated by simultaneous atomic force microscopy and fluorescence imaging [J].

Burns, AR .

LANGMUIR, 2003, 19 (20) :8358-8363

[9] Dehydration damage of domain-exhibiting supported bilayers: An AFM study on the protective effects of disaccharides and other stabilizing substances [J].

Chiantia, S ;

Kahya, N ;

Schwille, P .

LANGMUIR, 2005, 21 (14) :6317-6323

[10] Role of sphingomyelinase and ceramide in modulating rafts: do biophysical properties determine biologic outcome? [J].

Cremesti, AE ;

Goni, FM ;

Kolesnick, R .

FEBS LETTERS, 2002, 531 (01) :47-53

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