Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses

被引：15

作者：

Kasolo, F

Lishimpi, K

Chintu, C

Mwaba, P

Mudenda, V

Maswahu, D

Terunuma, H

Fletcher, H

Nunn, A

Lucas, S

Zumla, A

机构：

[1] Royal Free & UCL, Windeyer Inst Med Sci, Ctr Infect Dis & Int Hlth, Sch Med, London W1P 4JF, England

[2] St Thomas Kings & Guys Hosp, UMDS, Dept Pathol, Sch Med, London, England

[3] UCL, Hosp Trust, Med Res Council, Clin Trials Unit, London, England

[4] UNZA, UCLMS, TB HIV Res & Training Project, Luwaka, Zambia

[5] Univ Teaching Hosp, Dept Pathol & Microbiol, JICA Infect Dis Project, Luwaka, Zambia

来源：

JOURNAL OF PEDIATRICS | 2002年 / 140卷 / 03期

关键词：

D O I：

10.1067/mpd.2002.122468

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Polymerase chain reaction for Pneumocystis carinii DNA was performed on necropsy lung samples from children by means of P carinii-specific primers. P carinii DNA was identified in 22 of 22 (100%) samples with histologically proven P carinii pneumonia and 13 of 75 (17%) with non-P carinii pneumonia respiratory illness (sensitivity, 100%; specificity, 83%). The low specificity precludes the use of polymerase chain reaction as an alternative to histopathologic diagnosis.

引用

页码：367 / 369

页数：3

共 12 条

[1] PNEUMOCYSTIS-CARINII PNEUMONIA - AN UNCOMMON CAUSE OF DEATH IN AFRICAN PATIENTS WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME [J].