Retrograde support of neuronal survival without retrograde transport of nerve growth factor

被引：212

作者：

MacInnis, BL ^{[1
]}

Campenot, RB ^{[1
]}

机构：

[1] Univ Alberta, Dept Cell Biol, Edmonton, AB T6G 2H7, Canada

来源：

SCIENCE | 2002年 / 295卷 / 5559期

关键词：

D O I：

10.1126/science.1064913

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125-labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125-labeled NGF; whereas application of free iodine-125-labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.

引用

页码：1536 / 1539

页数：4

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