Development of a competitive enzyme-linked immunosorbent assay for diethylcarbamazine

被引:10
作者
Mitsui, Y
Takamura, N
Fujimaki, Y
Yamaguchi, T
Kitagawa, T
Aoki, Y
机构
[1] TANABE SEIYAKU CO LTD,ORGAN CHEM RES LAB,TODA,SAITAMA 335,JAPAN
[2] TANABE SEIYAKU CO LTD,BIOL RES LAB,TODA,SAITAMA 335,JAPAN
[3] NAGASAKI UNIV,FAC PHARMACEUT SCI,NAGASAKI 852,JAPAN
关键词
diethylcarbamazine; ELISA; serum concentration;
D O I
10.1046/j.1365-3156.1996.d01-77.x
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
A sensitive and reproducible competitive enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of diethylcarbamazine (DEC) in biological fluids was developed. Since DEC has no functional group to conjugate with bovine serum albumin (BSA), N-(2-aminoethyl)-N-ethyl-4-methyl-1-pipe (DEC-NH2) was first synthesized. This compound was then converted to carboxyl DEC (DEC-COOH) and conjugated to BSA and to poly-L-lysine for use as immunogen and sold-phase marker, respectively. The competitive ELISA was conducted by simultaneously incubating DEC with mouse anti-DEC antiserum over DEC-poly-L-lysine solid phase. Subsequently, the binding of anti-DEC antibody was detected by using sheep anti-mouse IgG peroxidase conjugate as a tracer. The reliability. determined by the coefficient of variation for inter and intra-assay, was satisfactory. The cross-reactivities of anti-DEC antibodies with DEC metabolites, related compounds and ivermectin were negligible. Using this assay, DEC levels were easily determined in serum of Mongolian jirds (Meriones unguiculatus) up to 4 hours following a single dose of DEC citrate base(100 mg/kg of body weight) via intraperitoneal route.
引用
收藏
页码:528 / 534
页数:7
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