Mining disease susceptibility genes through SNP analyses and expression profiling using MALDI-TOF mass spectrometry

被引:36
作者
Tang, K [1 ]
Oeth, P [1 ]
Kammerer, S [1 ]
Denissenko, MF [1 ]
Ekblom, J [1 ]
Jurinke, C [1 ]
van den Boom, D [1 ]
Braun, A [1 ]
Cantor, CR [1 ]
机构
[1] Sequenom Inc, San Diego, CA 92121 USA
关键词
single nucleotide polymorphism; gene expression; differential allele expression; pharmacogenomics; MALDI; mass spectrometry;
D O I
10.1021/pr034080s
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To find genes that underlie disease susceptibilities, genome-wide single nucleotide polymorphisms (SNPs) have been analyzed using high-throughput matrix assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). As a proof-of-concept for this approach, gene regions have been identified that were previously associated by others with certain diseases or traits. On the same technology platform, accurate and absolute transcriptional profiling can be performed and applied to allele expression analysis. Here, we provide a brief review of the technology and its applications to disease gene discovery.
引用
收藏
页码:218 / 227
页数:10
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