Comprehensive characterization of neuroblastoma cell line subtypes reveals bilineage potential similar to neural crest stem cells

被引：45

作者：

Acosta, Sandra ^{[1
]}

Lavarino, Cinzia ^{[1
]}

Paris, Raquel ^{[1
]}

Garcia, Idoia ^{[1
]}

de Torres, Carmen ^{[1
]}

Rodriguez, Eva ^{[1
]}

Beleta, Helena ^{[1
]}

Mora, Jaume ^{[1
]}

机构：

[1] Hosp St Joan Deu, Dev Tumor Biol Lab, Barcelona 08950, Spain

来源：

BMC DEVELOPMENTAL BIOLOGY | 2009年 / 9卷

关键词：

GENE-EXPRESSION; SCHWANN-CELLS; DIFFERENTIATION; TUMORS; BIOLOGY; ORIGIN; FAMILY; GROWTH; GLIA;

D O I：

10.1186/1471-213X-9-12

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Neuroblastic tumors (NBT) derive from neural crest stem cells (NCSC). Histologically, NBT are composed by neuroblasts and Schwannian cells. In culture, neuroblastic (N), substrate-adherent (S-) and intermediate phenotype (I-) cell subtypes arise spontaneously. Methods: Here, neuroblastoma (NB) cell line subtypes were characterized according to embryonic peripheral nervous system development markers (GAP43, Phox2b, Sox10, c-kit, GD2, NF68, vimentin, S100 beta, calcyclin and ABCG2), morphological features, gene expression and differentiation potential. I-type cells were investigated as a bipotential (neuronal and glial) differentiation stage. Results: Positive immunostaining of NCSC (GAP43, c-kit, NF68, vimentin and Phox2b) and undifferentiated cell (ABCG2) markers was observed in all NB subtypes. N- and I-type cells displayed cytoplasmic membrane GD2 staining, while nuclear calcyclin was restricted to S-type. Nand I-type cells showed similar phenotype and immunoreactivity pattern. Differential gene expression was associated with each cell subtype. N- and I-type cells displayed similar differentiation capacity towards neuronal and glial lineage fates. S-type cells, upon induction, did not show a neuronal-like phenotype, despite gene expression changes. Conclusion: Results suggest that N- and I-type NB cell subtypes represent an immature bilineage stage, able to progress towards neuronal and glial fates upon induction of differentiation. S-type cells appear irreversibly committed to a glial lineage fate.

引用

页数：14

共 45 条

[1] Role of ploidy, chromosome 1p, and Schwann cells in the maturation of neuroblastoma [J].

Ambros, IM ;

Zellner, A ;

Roald, B ;

Amann, G ;

Ladenstein, R ;

Printz, D ;

Gadner, H ;

Ambros, PF .

NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (23) :1505-1511

[2]

Barres B.A., 1997, Molecular and cellular approaches to neural development, P64

[3]

BIEDLER JL, 1973, CANCER RES, V33, P2643

[4] In neuroblastic tumours, Schwann cells do not harbour the genetic alterations of neuroblasts but may nevertheless share the same clonal origin [J].

Bourdeaut, F. ;

Ribeiro, A. ;

Paris, R. ;

Pierron, G. ;

Couturier, J. ;

Peuchmaur, M. ;

Delattre, O. .

ONCOGENE, 2008, 27 (21) :3066-3071

[5] DISIALOGANGLIOSIDES-GD2 AND GD3 ARE INVOLVED IN THE ATTACHMENT OF HUMAN-MELANOMA AND NEUROBLASTOMA-CELLS TO EXTRACELLULAR-MATRIX PROTEINS [J].

CHERESH, DA ;

PIERSCHBACHER, MD ;

HERZIG, MA ;

MUJOO, K .

JOURNAL OF CELL BIOLOGY, 1986, 102 (03) :688-696

[6]

Cheung IY, 2001, CLIN CANCER RES, V7, P1698

[7]

CICCARONE V, 1989, CANCER RES, V49, P219

[8] Role of all-Trans retinoic acid in neurite outgrowth and axonal elongation [J].

Clagett-Dame, Margaret ;

McNeill, Elizabeth M. ;

Muley, Parag D. .

JOURNAL OF NEUROBIOLOGY, 2006, 66 (07) :739-756

[9] Genome analysis and gene expression profiling of neuroblastoma and ganglioneuroblastoma reveal differences between neuroblastic and Schwannian stromal cells [J].

Coco, S ;

Defferrari, R ;

Scaruffi, P ;

Cavazzana, A ;

Di Cristofano, C ;

Longo, L ;

Mazzocco, K ;

Perri, P ;

Gambini, C ;

Moretti, S ;

Bonassi, S ;

Tonini, GP .

JOURNAL OF PATHOLOGY, 2005, 207 (03) :346-357

[10] A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].

DIMRI, GP ;

LEE, XH ;

BASILE, G ;

ACOSTA, M ;

SCOTT, C ;

ROSKELLEY, C ;

MEDRANO, EE ;

LINSKENS, M ;

RUBELJ, I ;

PEREIRASMITH, O ;

PEACOCKE, M ;

CAMPISI, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367

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