Surface modifying of microporous PTFE capillary for bilirubin removing from human plasma and its blood compatibility

被引：20

作者：

Jin, Gu ^{[1
]}

Yao, Qizhi ^{[1
]}

Zhang, Shanzi ^{[1
]}

Zhang, Lei ^{[1
,2
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China

[2] AnHui Entry Exit Inspect & Quarantine Bur, Hefei 230001, Peoples R China

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 08期

基金：

美国国家科学基金会;

关键词：

Polyvinylalcohol; Surface modification; Polytetrafluoroethylene; Endotoxin (bilirubin); Blood compatibility;

D O I：

10.1016/j.msec.2008.04.008

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this study, human serum albumin (HSA) was covalently immobilized onto the inner Surface of microporous poly(tetrafluoroethylene) (MPTFE) capillaries for direct bilirubin removal from human plasma. To obtain active binding sites for HSA, the MPTFE capillaries were chemically functionalized by using a coating of poly(vinyl alcohol) (PVA)-glycidyl methacrylate (GMA) copolymers. Characterization of grafted MPTFE capillaries was verified by XPS, Fourier transform infrared spectroscopy (FT-IR), scanning electronic microscopy (SEM). Non-specific adsorption on the PVA-GMA coated capillary remains low (<0.38 mg bilirubin/g), and higher affinity adsorption capacity, of up to 73.6 mg bilirubin/g polymer was obtained after HSA is immobilized. Blood compatibility of the grafted MPTFE capillary was evaluated by SEM and platelet rich plasma (PRP) contacting experiments. The experimental data on blood compatibility indicated that PVA-coated and PVA-GMA-HSA coated PTFE capillary showed a sharp suppress on platelets adhesion. The proposed method has the potential of serving in bilirubin removal in clinical application. (c) 2008 Elsevier B.V. All rights reserved

引用

页码：1480 / 1488

页数：9

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