Interaction of protein kinase C zeta with ZIP, a novel protein kinase c-binding protein

被引:188
作者
Puls, A
Schmidt, S
Grawe, F
Stabel, S
机构
[1] MAX PLANCK GESELL,MAX DELBRUCK LAB,D-50829 COLOGNE,GERMANY
[2] UNIV COLOGNE,INST ENTWICKLUNGSPHYSIOL,D-50923 COLOGNE,GERMANY
关键词
D O I
10.1073/pnas.94.12.6191
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The atypical protein kinase C (PMC) member PKC-zeta has been implicated in several signal transduction pathways regulating differentiation, proliferation or apoptosis of mammalian cells, We report here the identification of a cytoplasmic and membrane-associated protein that we name zeta-interacting protein (ZIP) and that interacts with the regulatory domain of PKC-zeta but not classic PKCs, The structural motifs in ZIP include a recently defined ZZ zinc finger as a potential protein binding module, two PEST sequences and a novel putative protein binding motif with the consensus sequence YXDEDX5SDEE/D. ZIP binds to the pseudosubstrate region in the regulatory domain of PKC-zeta and is phosphorylated by PKC-zeta in vitro. ZIP dimerizes via the same region that promotes binding to PKC-zeta suggesting a competitive situation between ZIP:ZIP and ZIP:PKC-zeta complexes, In the absence of PKC-zeta proper subcellular localization of ZIP is impaired and we show that intracellular targeting of ZIP is dependent on a balanced interaction with PKC-zeta. Taking into account the recent isolation of ZIP by others in different contexts we propose that ZIP may function as a scaffold protein linking PKC-zeta to protein tyrosine kinases and cytokine receptors.
引用
收藏
页码:6191 / 6196
页数:6
相关论文
共 50 条
  • [1] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [2] PROTEIN KINASE-C-ZETA ISOFORM IS CRITICAL FOR MITOGENIC SIGNAL-TRANSDUCTION
    BERRA, E
    DIAZMECO, MT
    DOMINGUEZ, I
    MUNICIO, MM
    SANZ, L
    LOZANO, J
    CHAPKIN, RS
    MOSCAT, J
    [J]. CELL, 1993, 74 (03) : 555 - 563
  • [3] Evidence for a role of MEK and MAPK during signal transduction by protein kinase C zeta
    Berra, E
    DiazMeco, MT
    Lozano, J
    Frutos, S
    Municio, MM
    Sanchez, P
    Sanz, L
    Moscat, J
    [J]. EMBO JOURNAL, 1995, 14 (24) : 6157 - 6163
  • [4] CARNERO A, 1995, ONCOGENE, V11, P1541
  • [5] COOPERATIVE INTERACTION OF S-POMBE PROTEINS REQUIRED FOR MATING AND MORPHOGENESIS
    CHANG, EC
    BARR, M
    WANG, Y
    JUNG, V
    XU, HP
    WIGLER, MH
    [J]. CELL, 1994, 79 (01) : 131 - 141
  • [6] OVEREXPRESSION OF MAMMALIAN PROTEIN-KINASE C-ZETA DOES NOT AFFECT THE GROWTH-CHARACTERISTICS OF NIH 3T3 CELLS
    CRESPO, P
    MISCHAK, H
    GUTKIND, JS
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 213 (01) : 266 - 272
  • [7] A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes
    Devergne, O
    Hummel, M
    Koeppen, H
    LeBeau, MM
    Nathanson, EC
    Kieff, E
    Birkenbach, M
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (02) : 1143 - 1153
  • [8] DiazMeco MT, 1996, MOL CELL BIOL, V16, P105
  • [9] The product of par-4, a gene induced during apoptosis, interacts selectively with the atypical isoforms of protein kinase C
    DiazMeco, MT
    Municio, MM
    Frutos, S
    Sanchez, P
    Lozano, J
    Sanz, L
    Moscat, J
    [J]. CELL, 1996, 86 (05) : 777 - 786
  • [10] PHOSPHOLIPID SIGNALING
    DIVECHA, N
    IRVINE, RF
    [J]. CELL, 1995, 80 (02) : 269 - 278