Paramagnetic metal ions in ligand screening:: The CoII matrix metalloproteinase 12

被引：46

作者：

Bertini, I

Fragai, M

Lee, YM

Luchinat, C

Terni, B

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Protera Srl, I-50019 Sesto Fiorentino, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 17期

关键词：

cobalt; drug research; enzymes; metalloproteins; NMR spectroscopy; zinc;

D O I：

10.1002/anie.200353453

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Smaller amounts of proteins are now required to screen for weak binding interactions between ligands and the active site of metalloproteins as a result of the substitution of diamagnetic zinc(II) with paramagnetic cobalt(II) in the matrix metalloproteinase MMP-12. 1H NMR spectroscopy then provides qualitative information on the orientation of the ligand in the catalytic site targeted by drugs (see picture).

引用

页码：2254 / 2256

页数：3

共 16 条

[1] Expression and high yield production of the catalytic domain of matrix metalloproteinase 12 and of an active mutant with increased solubility
Banci, L
Bertini, I
Ciulli, A
Fragai, M
Luchinat, C
Terni, B
[J]. JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 2003, 204 : 401 - 408
[2] X-ray structures of binary and ternary enzyme-product-Inhibitor complexes of matrix metalloproteinases
Bertini, I
Calderone, V
Fragai, M
Luchinat, C
Mangani, S
Terni, B
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (23) : 2673 - 2676
[3] Bertini I, 1984, Adv Inorg Biochem, V6, P71
[4] Bertini I, 2003, ANGEW CHEM, V115, P2777
[5] Bertini I, 2001, Solution NMR of Paramagnetic Molecules, V2
[6] One-dimensional relaxation- and diffusion-edited NMR methods for screening compounds that bind to macromolecules
Hajduk, PJ
Olejniczak, ET
Fesik, SW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (50) : 12257 - 12261
[7] Second-site NMR screening with a spin-labeled first ligand
Jahnke, W
Perez, LB
Paris, CG
Strauss, A
Fendrich, G
Nalin, CM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (30) : 7394 - 7395
[8] Spin label enhanced NMR screening
Jahnke, W
Rüdisser, S
Zurini, M
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (13) : 3149 - 3150
[9] Jahnke W., 2003, BioNMR in Drug Research, P341
[10] Substrate specificity determinants of human macrophage elastase (MMP-12) based on the 1.1 Å crystal structure
Lang, R
Kocourek, A
Braun, M
Tschesche, H
Huber, R
Bode, W
Maskos, K
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 312 (04) : 731 - 742

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