Chemo-affinity of titania for the column-switching HPLC analysis of phosphopeptides

被引：84

作者：

Sano, A ^{[1
]}

Nakamura, H ^{[1
]}

机构：

[1] Sci Univ Tokyo, Fac Pharmaceut Sci, Noda, Chiba 2788510, Japan

来源：

ANALYTICAL SCIENCES | 2004年 / 20卷 / 03期

关键词：

D O I：

10.2116/analsci.20.565

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A column-switching high-performance liquid chromatography (HPLC) system for the determination of phosphopeptides has been developed. The method is based on the selective adsorption of phosphopeptides on a titania (TiO2) precolumn and successive HPLC separation of the phosphopeptides on an anion-exchange column with a UV detector (215 nm). The recoveries of phosphopeptides were tested using authentic phosphopeptides [Gln-Ile-Ser(p)-Val-Arg, Ile-Ser(p)-Val-Arg and Lys-Gln-Ile-Ser(p)-Val-Arg] at an injection amount of 1 mug. The recoveries were 74.3, 79.6, and 82.6%, respectively, while the corresponding dephosphopeptides were not retained on the titania precolumn.

引用

页码：565 / 566

页数：2

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