Mouse cholecystokinin type-A receptor gene and its structural analysis

被引：20

作者：

Takata, Y

Takiguchi, S

Kataoka, K

Funakoshi, A

Miyasaka, K

Kono, A

机构：

[1] KYUSHU NATL CANC CTR, DIV CHEMOTHERAPY, FUKUOKA 815, JAPAN

[2] KYUSHU NATL CANC CTR, DIV GASTROENTEROL, FUKUOKA 815, JAPAN

[3] TOKYO METROPOLITAN INST GERONTOL, DEPT CLIN PHYSIOL, TOKYO 173, JAPAN

来源：

GENE | 1997年 / 187卷 / 02期

关键词：

G protein-coupled receptor; gene structure; cDNA sequence; alternative splicing;

D O I：

10.1016/S0378-1119(96)00765-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mouse cholecystokinin type-A receptor (CCK(A)R) gene was cloned and sequenced, and the exon/intron boundaries were determined by cDNA cloning. The gene, approximately 10 kb in length, contains the entire coding region, and consists of five exons. The deduced amino acid sequence was homologous with that of other species, with the exception of an additional DNA sequence encoding 7 amino acids in exon 5. A region of the 5' end of exon 2 appeared to be alternatively spliced, and generated an isoform shorter by 52 bases. The shorter isoform may encode an 48 amino acid open reading frame due to frameshift of translation. These two mRNA isoforms were expressed equally in the mouse gallbladders.

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页码：267 / 271

页数：5

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