Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR

被引：20

作者：

Respess, RA

Butcher, A

Wang, H

Chaowanachan, T

Young, N

Shaffer, N

Mastro, TD

Biryahwaho, B

Downing, R

Tanuri, A

Schechter, M

Pascu, R

Zekeng, L

Kaptue, L

Gurtler, L

Eberle, J

Ellenberger, D

Fridlund, C

Rayfield, M

Kwok, S

机构：

[1] ROCHE MOL SYST INC,ALAMEDA,CA 94501

[2] ROCHE MOL SYST INC,SOMERVILLE,NJ 08876

[3] HIV AIDS COLLABORAT,NONTHABURI,THAILAND

[4] UGANDA VIRUS RES INST,ENTEBBE,UGANDA

[5] UNIV FED RIO DE JANEIRO,RIO JANEIRO,BRAZIL

[6] UNIV MED & PHARM,TIRGU MURES,ROMANIA

[7] CHU YAOUNDE,YAOUNDE,CAMEROON

[8] MAX VON PETTENKOFER INST,D-8000 MUNICH,GERMANY

[9] CTR DIS CONTROL & PREVENT,HIV LAB,INVEST BRANCH,ATLANTA,GA 30333

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 05期

关键词：

D O I：

10.1128/JCM.35.5.1284-1286.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.

引用

页码：1284 / 1286

页数：3

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