Mucin gene expression in the rat middle ear: An improved method for RNA harvest

被引：19

作者：

Lin, JZ

Ho, S

Shekels, L

Paparella, MM

Kim, Y

机构：

[1] Univ Minnesota, Dept Otolaryngol, Otitis Med Res Ctr, Sch Med, Minneapolis, MN 55455 USA

[2] Vet Adm Med Ctr, Dept Med, Minneapolis, MN 55417 USA

[3] Univ Minnesota, Dept Pediat, Otitis Med Res Ctr, Sch Med, Minneapolis, MN 55455 USA

来源：

ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY | 1999年 / 108卷 / 08期

关键词：

middle ear epithelial cells; MUC2 mucin gene; Sprague-Dawley rats;

D O I：

10.1177/000348949910800809

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Mucins are heavily glycosylated proteins characterized by high molecular weight and heterogeneous structure. Mucin genes are expressed in a tissue- or epithelium-specific manner. Although mucins are known to be important structural components of the mucociliary transport system that protects epithelium against invading microorganisms, very little is known about mucin gene expression unique to the middle ear. This study demonstrated that middle ear messenger RNA specifically hybridized with rat MUC2 and human MUC2 (SMUC-41) complementary DNA probes. MUC3 and MUC5AC mucin genes, dominantly expressed in rodent intestine and trachea, were not detected in the rat middle ears in this study. The middle ear MUC2 messenger RNA harvested by lavage was characterized by a single transcript - unlike its counterpart in intestine and airways, which is characterized by polydispersity - suggestive of a better method for RNA analysis. It was concluded that rat middle ears possess a MUC2 mucin gene or homologue of human MUC2 (SMUC-41).

引用

页码：762 / 768

页数：7

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