Differential expression of syntrophins and analysis of alternatively spliced dystrophin transcripts in the mouse brain

被引:43
作者
Gorecki, DC
Abdulrazzak, H
Lukasiuk, K
Barnard, EA
机构
[1] ROYAL FREE HOSP,SCH MED,MOL NEUROBIOL UNIT,LONDON,ENGLAND
[2] ROYAL FREE HOSP,SCH MED,DEPT CLIN GENET,LONDON,ENGLAND
[3] M NENCKI INST EXPT BIOL,TISSUE CULTURE UNIT,PL-02093 WARSAW,POLAND
基金
英国惠康基金;
关键词
alternative splicing; dystrophin; dystrophin-associated protein complex; mouse brain; syntrophin; syntrophin-binding site;
D O I
10.1111/j.1460-9568.1997.tb01447.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Expression of syntrophin genes, encoding members of the dystrophin-associated protein complex, was studied in the mouse brain, In the hippocampal formation there is distinctive co-localization of specific syntrophins with certain dystrophin isoforms in neurons, e.g. alpha(1)-syntrophin with the C-dystrophin in CA regions and beta(2)-syntrophin with the G-dystrophin in the dentate gyrus. Expression of the alpha(1)-syntrophin is predominant in CA regions and the olfactory bulb and it is also present in the cerebral cortex and the dentate gyrus. The beta(2)-syntrophin mRNA is most abundant in the dentate gyrus and is also evident in the pituitary, the cerebral cortex and in Ammon's horn and in traces in the caudate putamen. The choroid plexus was labelled by both alpha(1)- and beta(2)-syntrophin-specific probes. The expression of syntrophins in the brain correlates with expression of dystrophins and dystroglycan. There are brain areas such as the cerebral cortex where several different syntrophins and dystrophins are expressed together, Syntrophin expression co-localizes with utrophin in the choroid plexus and caudate putamen, Finally, no syntrophin was detected in the cerebellar Purkinje cells where the specific dystrophin isoform (P-type) is present, This specific distribution of syntrophins in the brain is particularly interesting, as muscle syntrophin interacts with neuronal nitric oxide synthase. This may suggest that the dystrophin-associated protein complex may be involved in synaptic organisation and signal transduction machinery in both muscle and neurons. The dystrophin isoform, with exons 71-74 spliced out and hence lacking syntrophin binding sites, had been believed to be predominant in the brain, but our analyses using in situ hybridization, S1 nuclease protection and the semi-quantitative polymerase chain reaction revealed that this alternatively spliced mRNA is a minor, tow abundance form in the brain.
引用
收藏
页码:965 / 976
页数:12
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