Characteristics of hyperpolarization-activated cation currents in portal vein smooth muscle cells

被引:24
作者
Greenwood, IA [1 ]
Prestwich, SA [1 ]
机构
[1] St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, London SW17 0RE, England
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2002年 / 282卷 / 04期
关键词
vascular smooth muscle; membrane hyperpolarization;
D O I
10.1152/ajpcell.00393.2001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Voltage-clamp studies of freshly isolated smooth muscle cells from rabbit portal vein revealed the existence of a time-dependent cation current evoked by membrane hyperpolarization (termed I-h). Both the rate of activation and the amplitude of I-h were enhanced by membrane hyperpolarization. Half-maximal activation of I-h was about -105 mV with conventional whole cell and -80 mV when the perforated patch technique was used. In current clamp, injection of hyperpolarizing current produced a marked depolarizing "sag" followed by rebound depolarization. Activation of I-h was augmented by an increase in the extracellular K+ concentration and was blocked rapidly by externally applied Cs+ (1-5 mM). The bradycardic agent ZD-7288 (10 muM), a selective inhibitor of I-h, produced a characteristically slow inhibition of the portal vein Ih. The depolarizing sag recorded in current clamp was also abolished by application of 5 mM Cs+. Cs+ significantly decreased the frequency of spontaneous contractions in both whole rat portal vein and rabbit portal vein segments. Multiplex RT-PCR of rabbit portal vein myocytes using primers derived from existing genes for hyperpolarization-activated cation channels (HCN1-4) revealed the existence of cDNA clones corresponding to HCN2, 3, and 4. The present study shows that portal vein myocytes contain genes shown to encode for hyperpolarization-activated channels and exhibit an endogenous current with characteristics similar to I-h in other cell types. This conductance appears to determine, in part, the rhythmicity of this vessel.
引用
收藏
页码:C744 / C753
页数:10
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