Molecular identification of mycorrhizal fungi by direct amplification of microsatellite regions

被引:93
作者
Longato, S [1 ]
Bonfante, P [1 ]
机构
[1] UNIV TURIN, DIPARTIMENTO BIOL VEGETALE, I-10125 TURIN, ITALY
来源
MYCOLOGICAL RESEARCH | 1997年 / 101卷
关键词
D O I
10.1017/S0953756296002766
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have screened the genome of ecto- and endo-mycorrhizal fungi by using primers designed on microsatellite sequences: (CT)(8), (CA)(8), (GACA)(4), (TGTC)(4), (GTG)(5). PCR experiments proved that microsatellites such as (GTG)(5) exist as short repeated sequences in II species of Tuber (Ascomycetes) and seven species within Glomales (Zygomycetes). Variations in the banding pattern obtained by DNA fingerprinting enabled all these species and some isolates to be distinguished according to the number, size and intensity of the fragments. (GACA)(4) and (TGTC)(4) also led to successful amplifications in some isolates from Tuber and Glomales. These experiments demonstrate that microsatellite primers are reliable, sensitive and technically simple tools for assaying genetic variability in mycorrhizal fungi, and can be used to discriminate mycorrhizal symbionts with different taxonomic features. (GTG)(5) in fact led to species-specific fingerprints in both truffles, which are closely related species, and in Glomales, which are quite separate species in evolutionary terms.
引用
收藏
页码:425 / 432
页数:8
相关论文
共 38 条
  • [1] ANALYSIS OF THE CELL-CYCLE IN AN ARBUSCULAR MYCORRHIZAL FUNGUS BY FLOW-CYTOMETRY AND BROMODEOXYURIDINE LABELING
    BIANCIOTTO, V
    BARBIERO, G
    BONFANTE, P
    [J]. PROTOPLASMA, 1995, 188 (3-4) : 161 - 169
  • [2] BONFANTE P, 1996, MYCORRHIZAS INTEGRAT, P163
  • [3] HIGH-RESOLUTION OF HUMAN EVOLUTIONARY TREES WITH POLYMORPHIC MICROSATELLITES
    BOWCOCK, AM
    RUIZLINARES, A
    TOMFOHRDE, J
    MINCH, E
    KIDD, JR
    CAVALLISFORZA, LL
    [J]. NATURE, 1994, 368 (6470) : 455 - 457
  • [4] INTEGRATIVE TRANSFORMATION OF A ZYGOMYCETE, ABSIDIA-GLAUCA, WITH VECTORS CONTAINING REPETITIVE DNA
    BURMESTER, A
    WOSTEMEYER, A
    WOSTEMEYER, J
    [J]. CURRENT GENETICS, 1990, 17 (02) : 155 - 161
  • [5] DNA-SEQUENCING WITH ARBITRARY PRIMES PAIRS
    BURT, A
    CARTER, DA
    WHITE, TJ
    TAYLOR, JW
    [J]. MOLECULAR ECOLOGY, 1994, 3 (05) : 523 - 525
  • [6] DNA polymorphism in morels: PCR/RFLP analysis of the ribosomal DNA spacers and microsatellite-primed PCR
    Buscot, F
    Wipf, D
    DeBattista, C
    Munch, JC
    Botton, B
    Martin, F
    [J]. MYCOLOGICAL RESEARCH, 1996, 100 : 63 - 71
  • [7] Ceruti A, 1960, ICONOGRAPHIA MYCO S2, V28
  • [8] Inter- and intraspecific variation within the morphologically similar arbuscular mycorrhizal fungi Glomus mosseae and Glomus coronatum
    Dodd, JC
    Rosendahl, S
    Giovannetti, M
    Broome, A
    Lanfranco, L
    Walker, C
    [J]. NEW PHYTOLOGIST, 1996, 133 (01) : 113 - 122
  • [9] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION
    DON, RH
    COX, PT
    WAINWRIGHT, BJ
    BAKER, K
    MATTICK, JS
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (14) : 4008 - 4008
  • [10] Long, polymorphic microsatellites in simple organisms
    Field, D
    Wills, C
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1996, 263 (1367) : 209 - 215