Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates

被引：9

作者：

Allen, CCR

Walker, CE

Sharma, ND

Kerley, NA

Boyd, DR

Dalton, H ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

[2] Queens Univ Belfast, QUESTOR Ctr, Belfast BT9 5AG, Antrim, North Ireland

[3] Queens Univ Belfast, Sch Chem, Belfast BT9 5AG, Antrim, North Ireland

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2002年 / 20卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Pseudomonas putida ML2; biotransformations; biocatalysis; benzene cis-dihydrodiol dehydrogenase;

D O I：

10.1080/10242420290029454

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enantiopure (1S, 2S)-cis-dihydrodiol metabolites 2B-5B have been obtained in low yield from the corresponding monosubstituted halobenzene substrates 2A-5A, using a wild-type strain of Pseudomonas putida (ML2) containing benzene dioxygenase (BDO). Benzene cis-dihydrodiol dehydrogenase (BCD) from P. putida ML2 and naphthalene cis-dihydrodiol dehydrogenase (NCD) from P. putida 8859 were purified and used in a comparative study of the stereoselective biotransformation of cis-dihydrodiol enantiomers 2B-5B. The BCD and NCD enzymes were found to accept cis-dihydrodiol enantiomers of monosubstituted benzene cis-dihydrodiol substrates 2B-5B of opposite absolute configuration. The acyclic alkene 1,2-diols 10-17 were also found to be acceptable substrates for BCD.

引用

页码：257 / 264

页数：8

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