Integration of hepadnavirus DNA in infected liver: Evidence for a linear precursor

被引：105

作者：

Yang, WG ^{[1
]}

Summers, J ^{[1
]}

机构：

[1] Univ New Mexico, Dept Mol Genet & Microbiol, Albuquerque, NM 87131 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 12期

关键词：

D O I：

10.1128/JVI.73.12.9710-9717.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA of the avian hepadnavirus, duck hepatitis B virus, was found to be integrated at low abundance into the cellular DNA extracted from the livers of infected ducklings. The frequency of integration was estimated to be at least one viral genome per 10(3) to 10(4) cells by 6 days postinfection. The structures of virus-cell junctions determined by sequencing were compared with those of virus-virus junctions formed by nonhomologous recombination between the ends of linear viral DNA forms. This comparison allowed us to conclude that linear viral DNA was the preferential form used as an integration substrate. Potential factors promoting viral DNA integration during chronic infection are discussed.

引用

页码：9710 / 9717

页数：8

共 32 条

[1] Naturally occurring mutations define a novel function of the hepatitis B virus core promoter in core protein expression [J].

Baumert, TF ;

Marrone, A ;

Vergalla, J ;

Liang, TJ .

JOURNAL OF VIROLOGY, 1998, 72 (08) :6785-6795

[2] A novel transcriptional element in circular DNA monomers of the duck hepatitis B virus [J].

BeckelMitchener, A ;

Summers, J .

JOURNAL OF VIROLOGY, 1997, 71 (10) :7917-7922

[3] CHRONIC HEPATITIS IN HBSAG-CARRIERS WITH SERUM HBV-DNA AND ANTI-HBE [J].

BONINO, F ;

ROSINA, F ;

RIZZETTO, M ;

RIZZI, R ;

CHIABERGE, E ;

TARDANICO, R ;

CALLEA, F ;

VERME, G .

GASTROENTEROLOGY, 1986, 90 (05) :1268-1273

[4]

Buendia M A, 1992, Semin Cancer Biol, V3, P309

[5] HEPATITIS-B VIRUSES AND HEPATOCELLULAR-CARCINOMA [J].

BUENDIA, MA .

ADVANCES IN CANCER RESEARCH, 1992, 59 :167-226

[6] HEPATITIS-B VIRUS IMMUNOPATHOLOGY [J].

CHISARI, FV ;

FERRARI, C .

SPRINGER SEMINARS IN IMMUNOPATHOLOGY, 1995, 17 (2-3) :261-281

[7] FREQUENT ACTIVATION OF N-MYC GENES BY HEPADNAVIRUS INSERTION IN WOODCHUCK LIVER-TUMORS [J].

FOUREL, G ;

TREPO, C ;

BOUGUELERET, L ;

HENGLEIN, B ;

PONZETTO, A ;

TIOLLAIS, P ;

BUENDIA, MA .

NATURE, 1990, 347 (6290) :294-298

[8]

GANEM D, 1996, VIROLOGY, P2703

[9] Double-stranded linear duck hepatitis B virus (DHBV) stably integrates at a higher frequency than wild-type DHBV in LMH chicken hepatoma cells [J].

Gong, SS ;

Jensen, AD ;

Chang, CJ ;

Rogler, CE .

JOURNAL OF VIROLOGY, 1999, 73 (02) :1492-1502

[10] DUCK HEPATITIS-B VIRUS INTEGRATIONS IN LMH CHICKEN HEPATOMA-CELLS - IDENTIFICATION AND CHARACTERIZATION OF NEW EPISOMALLY DERIVED INTEGRATIONS [J].

GONG, SS ;

JENSEN, AD ;

WANG, HP ;

ROGLER, CE .

JOURNAL OF VIROLOGY, 1995, 69 (12) :8102-8108

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