Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125

被引：19

作者：

Takami, H ^{[1
]}

Takaki, Y ^{[1
]}

Nakasone, K ^{[1
]}

Sakiyama, T ^{[1
]}

Maeno, G ^{[1
]}

Sasaki, R ^{[1
]}

Hirama, C ^{[1
]}

Fuji, F ^{[1
]}

Masui, N ^{[1
]}

机构：

[1] Japan Marine Sci & Technol Ctr, Deep Sea Microorganisms REs Grp, Yokosuka, Kanagawa 2370061, Japan

来源：

EXTREMOPHILES | 1999年 / 3卷 / 03期

关键词：

alkaliphile; Bacillus halodurans; C-125; genetic map; genome analysis;

D O I：

10.1007/s007920050120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seventeen Sse8387I linking clones isolated from the chromosome of Bacillus halodurans C-125 for the purpose of constructing a physical map were sequenced and analyzed by comparison with the BSORF database and the nonredundant protein databank, The orientations of Sse8387I or AscI linking clones serving to join adjacent fragments were determined by southern blot analysis using specific DNA probes. One-third of the open reading frames (ORFs) identified in the Sse8387I linking clones showed no significant similarity to any protein so far reported. The ORFs showing significant similarities to those of Bacillus subtilis were mapped in the chromosome of strain C-125, and the locations of the putative genes on the map were not well conserved between B. halodurans C-125 and B. subtilis.

引用

页码：227 / 233

页数：7

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