Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

被引：24

作者：

deAlmeida, PJLP

Ndao, M

VanMeirvenne, N

Geerts, S

机构：

来源：

ACTA TROPICA | 1997年 / 66卷 / 01期

关键词：

Trypanosoma vivax; goat; diagnosis; PCR; Chelex(R) 100;

D O I：

10.1016/S0001-706X(97)00677-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex(R) 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques. (C) 1997 Elsevier Science B.V.

引用

页码：45 / 50

页数：6

共 8 条

[1]

BEUTLER E, 1990, BIOTECHNIQUES, V9, P166

[2] TRYPANOSOMA-BRUCEI - RAPID MATCHING METHOD FOR ESTIMATING HOSTS PARASITEMIA [J].