Atomic Force Microscopy Combined with Optical Microscopy for Cells Investigation

被引：47

作者：

Cascione, Mariafrancesca ^{[1
,2
]}

De Matteis, Valeria ^{[1
]}

Rinaldi, Rosaria ^{[1
,2
]}

Leporatti, Stefano ^{[3
]}

机构：

[1] Univ Salento, Dipartimento Matemat & Fis Ennio De Giorgi, Via Monteroni, I-73100 Lecce, Italy

[2] CNR, Ist Nanosci, Euromediterranean Ctr Nanomat Modelling & Technol, Via Arnesano 16, Lecce, Italy

[3] Ist Nanotecnol, CNR Nantotec, Polo Nanotecnol, C-O Campus Ecoteckne,Via Monteroni, I-73100 Lecce, Italy

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2017年 / 80卷 / 01期

关键词：

atomic force microscopy; cells; fluorescence microscopy; confocal laser scanning microscopy; total internal reflection fluorescence microscopy; INTERNAL-REFLECTION FLUORESCENCE; BREAST-CANCER CELLS; ENDOTHELIAL-CELLS; LIVING CELLS; REAL-TIME; MEMBRANE; ACTIN; AFM; SURFACE; MECHANOTRANSDUCTION;

D O I：

10.1002/jemt.22696

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100123 [人体微生态学]; 100210 [外科学];

摘要：

This review reports on the combined use of the atomic force microscopy (AFM) and several type of optical/fluorescence/laser scanning microscopy for investigating cells. It is shown that the hybrid systems of AFM with optical-derived microscopies enable to study in detail cell surface properties (such as topography), their mechanical properties (e.g., Young's modulus) mechanotransduction phenomena and allow to gain insight into biological-related pathways and mechanisms in the complex nanoworld of cells. (C) 2016 Wiley Periodicals, Inc.

引用

页码：109 / 123

页数：15

共 46 条

[1]

CELL-SUBSTRATE CONTACTS ILLUMINATED BY TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].