Efficient pseudotyping of murine leukemia virus particles with chimeric human foamy virus envelope proteins

被引：84

作者：

Lindemann, D ^{[1
]}

Bock, M ^{[1
]}

Schweizer, M ^{[1
]}

Rethwilm, A ^{[1
]}

机构：

[1] INST MED MIKROBIOL & HYG,ABT VIROL,D-79104 FREIBURG,GERMANY

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 06期

关键词：

D O I：

10.1128/JVI.71.6.4815-4820.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Incorporation of human foamy virus (HFV) envelope proteins into murine leukemia virus (MuLV) particles was studied in a transient transfection packaging cell system. We report here that wild-type HFV envelope protein can pseudotype MuLV particles, albeit at low efficiency. Complete or partial removal of the HFV cytoplasmic tail resulted in an abolishment or reduction of HFV-mediated infectivity, implicating a role of the HFV envelope cytoplasmic tail in the pseudotyping of MuLV particles. Mutation of the endoplasmic reticulum retention signal present in the HFV envelope cytoplasmic tail did not result in a higher relative infectivity of pseudotyped retroviral vectors. However, a chimeric envelope protein, containing an unprocessed MuLV envelope cytoplasmic domain fused to a truncated HFV envelope protein, showed an enhanced HFV specific infectivity as a result of an increased incorporation of chimeric envelope proteins into MuLV particles.

引用

页码：4815 / 4820

页数：6

共 40 条

[1]

TRANSDUCTION OF PRIMARY HUMAN HEPATOCYTES WITH AMPHOTROPIC AND XENOTROPIC RETROVIRAL VECTORS [J].

ADAMS, RM ;

SORIANO, HE ;

WANG, M ;

DARLINGTON, G ;

STEFFEN, D ;

LEDLEY, FD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :8981-8985

[2]

IMPROVED TRANSFER OF THE LEUKOCYTE INTEGRIN CD18 SUBUNIT INTO HEMATOPOIETIC-CELL LINES BY USING RETROVIRAL VECTORS HAVING A GIBBON APE LEUKEMIA-VIRUS ENVELOPE [J].

BAUER, TR ;

MILLER, AD ;

HICKSTEIN, DD .

BLOOD, 1995, 86 (06) :2379-2387

[3]

A COMPARATIVE-STUDY OF HIGHER PRIMATE FOAMY VIRUSES, INCLUDING A NEW VIRUS FROM A GORILLA [J].

BIENIASZ, PD ;

RETHWILM, A ;

PITMAN, R ;

DANIEL, MD ;

CHRYSTIE, I ;

MCCLURE, MO .

VIROLOGY, 1995, 207 (01) :217-228

[4]

CELL-CYCLE DEPENDENCE OF FOAMY RETROVIRUS INFECTION [J].

BIENIASZ, PD ;

WEISS, RA ;

MCCLURE, MO .

JOURNAL OF VIROLOGY, 1995, 69 (11) :7295-7299

[5]

Induction of antigen-specific tumor immunity by genetic and cellular vaccines against MAGE: Enhanced tumor protection by coexpression of granulocyte-macrophage colony-stimulating factor and B7-1 [J].

Bueler, H ;

Mulligan, RC .

MOLECULAR MEDICINE, 1996, 2 (05) :545-555

[6]

VESICULAR STOMATITIS-VIRUS G GLYCOPROTEIN PSEUDOTYPED RETROVIRAL VECTORS - CONCENTRATION TO VERY HIGH-TITER AND EFFICIENT GENE-TRANSFER INTO MAMMALIAN AND NONMAMMALIAN CELLS [J].

BURNS, JC ;

FRIEDMANN, T ;

DRIEVER, W ;

BURRASCANO, M ;

YEE, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8033-8037

[7]

GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[8]

Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitis virus by using a modified tetracycline inducible system [J].

Chen, ST ;

Iida, A ;

Guo, L ;

Friedmann, T ;

Yee, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (19) :10057-10062

[9]

ANALYSIS OF MUTATION IN HUMAN-CELLS BY USING AN EPSTEIN-BARR-VIRUS SHUTTLE SYSTEM [J].

DUBRIDGE, RB ;

TANG, P ;

HSIA, HC ;

LEONG, PM ;

MILLER, JH ;

CALOS, MP .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :379-387

[10]

Foamy virus reverse transcriptase is expressed independently from the Gag protein [J].

Enssle, J ;

Jordan, I ;

Mauer, B ;

Rethwilm, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4137-4141

← 1 2 3 4 →