Cellubrevin alterations and Mycobacterium tuberculosis phagosome maturation arrest

被引:45
作者
Fratti, RA
Chua, J
Deretic, V
机构
[1] Univ New Mexico, Sch Med, Dept Mol Genet & Microbiol, Albuquerque, NM 87131 USA
[2] Univ Michigan, Sch Med, Mol & Cellular Biol Program, Ann Arbor, MI USA
[3] Univ New Mexico, Sch Med, Dept Physiol & Cell Biol, Albuquerque, NM 87131 USA
关键词
D O I
10.1074/jbc.M200335200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The intracellular trafficking processes controlling phagosomal maturation remain to be fully delineated. Mycobacterium tuberculosis var. bovis BCG, an organism that causes phagosomal maturation arrest, has emerged as a tool for dissection of critical phagosome biogenesis events. In this work, we report that cellubrevin, a v-SNARE functioning in endosomal recycling and implicated in endosomal interactions with post-Golgi compartments, plays a role in phagosomal maturation and that it is altered on mycobacterial phagosomes. Both mycobacterial phagosomes, which undergo maturation arrest, and model phagosomes containing latex beads, which follow the normal pathway of maturation into phagolysosomes, acquired cellubrevin. However, the mycobacterial and model phagosomes differed, as a discrete proteolytic degradation of this SNARE was detected on mycobacterial phagosomes. The observed cellubrevin alteration on mycobacterial phagosomes was not a passive event secondary to a maturation arrest at another checkpoint of the phagosome maturation pathway, since pharmacological inhibitors of phagosomal/endosomal pathways blocking phagosomal maturation did not cause cellubrevin degradation on model phagosomes. Cellubrevin status on phagosomes had consequences on phagosomal membrane and lumenal content trafficking, involving plasma membrane marker recycling and delivery of lysosomal enzymes. These results suggest that cellubrevin plays a role in phagosomal maturation and that it is a target for modification by mycobacteria or by infection-induced processes in the host cell.
引用
收藏
页码:17320 / 17326
页数:7
相关论文
共 74 条
[1]   VAMP-7 mediates vesicular transport from endosomes to lysosomes [J].
Advani, RJ ;
Yang, B ;
Prekeris, R ;
Lee, KC ;
Klumperman, J ;
Scheller, RH .
JOURNAL OF CELL BIOLOGY, 1999, 146 (04) :765-775
[2]   Increased expression of Rab5a correlates directly with accelerated maturation of Listeria monocytogenes phagosomes [J].
Alvarez-Dominguez, C ;
Stahl, PD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (17) :11459-11462
[3]   Phagocytosed live Listeria monocytogenes influences rab5-regulated in vitro phagosome-endosome fusion [J].
AlvarezDominguez, C ;
Barbieri, AM ;
Beron, W ;
WandingerNess, A ;
Stahl, PD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (23) :13834-13843
[4]  
AlvarezDominguez C, 1997, J CELL SCI, V110, P731
[5]   RESPONSE OF CULTURED MACROPHAGES TO MYCOBACTERIUM-TUBERCULOSIS, WITH OBSERVATIONS ON FUSION OF LYSOSOMES WITH PHAGOSOMES [J].
ARMSTRONG, JA ;
HART, PD .
JOURNAL OF EXPERIMENTAL MEDICINE, 1971, 134 (03) :713-+
[6]   Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation [J].
Bajno, L ;
Peng, XR ;
Schreiber, AD ;
Moore, HP ;
Trimble, WS ;
Grinstein, S .
JOURNAL OF CELL BIOLOGY, 2000, 149 (03) :697-705
[7]   N-ethylmaleimide-sensitive factor acts at a profusion ATP-dependent step in Ca2+-activated exocytosis [J].
Banerjee, A ;
Barry, VA ;
DasGupta, BR ;
Martin, TFJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) :20223-20226
[8]   PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT [J].
BLOCK, MR ;
GLICK, BS ;
WILCOX, CA ;
WIELAND, FT ;
ROTHMAN, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7852-7856
[9]   Role of COPI in phagosome maturation [J].
Botelho, RJ ;
Hackam, DJ ;
Schreiber, AD ;
Grinstein, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (21) :15717-15727
[10]   Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation [J].
Bryant, NJ ;
James, DE .
EMBO JOURNAL, 2001, 20 (13) :3380-3388