Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation

被引：136

作者：

Arica, M. Yakup ^{[1
]}

Altintas, Begum ^{[2
]}

Bayramoglu, Guelay ^{[1
]}

机构：

[1] Gazi Univ, Fac Arts & Sci, Biochem Proc & Biomat Res Lab, TR-06500 Ankara, Turkey

[2] Gazi Univ, Inst Sci & Technol, Dept Environm Sci, TR-06570 Ankara, Turkey

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 02期

关键词：

Laccase; Immobilized enzyme; Enzyme technology; Enzyme reactor; Dye removal; COVALENT IMMOBILIZATION; GLYCIDYL METHACRYLATE; CATALYTIC-PROPERTIES; POLYPHENOL OXIDASE; REACTIVE MEMBRANES; MAGNETIC BEADS; STABILITY; POLYMERIZATION; SUPPORTS; TYROSINASE;

D O I：

10.1016/j.biortech.2008.07.038

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9 mg/g, respectively. The maximum activity (V-max) and Michaelis constant (K-m) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6 U/min and 0.47 mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：665 / 669

页数：5

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