Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake

被引：71

作者：

Gessesse, A

Gashe, BA

机构：

[1] Department of Biology, Addis Ababa University, Addis Ababa

[2] Department of Biological Sciences, University of Botswana, Gaborone

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 05期

关键词：

PURIFICATION;

D O I：

10.1023/A:1018308513853

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new alkaliphilic strain of Microbacterium producing an alkaline protease was isolated from an alkaline soda lake in Ethiopia. High level of protease activity was produced in the presence of glucose and sucrose as carbon sources. The optimum temperature and pH for activity were 65 degrees C and 9.5-11.5 respectively. Above 50 degrees C, Ca2+ was required for enzyme activity and stability. At 55 and 60 degrees C it retained 100 and 85% of its original activity respectively after 1 h incubation. The enzyme was stable over the pH range of 5-12.

引用

页码：479 / 481

页数：3

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