Induction of macrophage C-C chemokine expression by titanium alloy and bone cement particles

被引：116

作者：

Nakashima, Y ^{[1
]}

Sun, DH ^{[1
]}

Trindade, MCD ^{[1
]}

Chun, LE ^{[1
]}

Song, Y ^{[1
]}

Goodman, SB ^{[1
]}

Schurman, DJ ^{[1
]}

Maloney, WJ ^{[1
]}

Smith, RL ^{[1
]}

机构：

[1] Stanford Univ, Med Ctr, Orthopaed Res Lab, Sch Med, Stanford, CA 94305 USA

来源：

JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME | 1999年 / 81B卷 / 01期

关键词：

D O I：

10.1302/0301-620X.81B1.8884

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Particulate wear debris is associated with I periprosthetic inflammation and loosening in total joint arthroplasty, We tested the effects of titanium alloy (Ti-alloy) and PMMA particles on monocyte/macrophage expression of the C-C chemokines, monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory protein-1 alpha (MIP-1 alpha), and regulated upon activation normal T expressed and secreted protein (RANTES). Periprosthetic granulomatous tissue was analysed for expression of macrophage chemokines by immunohistochemistry. Chemokine expression in human monocytes/macrophages exposed to Ti-alloy and PMMA particles in vitro was determined by RT-PCR, ELISA and monocyte migration. We observed MCP-1 and MIP-1 alpha expression in all tissue samples from failed arthroplasties, Ti-alloy and PMMA particles increased expression of MCP-1 and MIP-1 alpha in macrophages in vitro in a dose- and time-dependent manner whereas RANTES was not detected. mRNA signal levels for MCP-1 and MIP-1 alpha were also observed in cells after exposure to particles. Monocyte migration was stimulated by culture medium collected from macrophages exposed to Ti-alloy and PMMA particles, Antibodies to MCP-1 and MIP-1 alpha: inhibited chemotactic activity of the culture medium samples. Release of C-C chemokines by macrophages in response to wear particles may contribute to chronic inflammation at the bone-implant interface in total joint arthroplasty.

引用

页码：155 / 162

页数：8

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