Lanthanide modulation of the orientation of macromolecules induced by purple membrane

被引：9

作者：

Bernadó, P

Barbieri, R

Padrós, E

Luchinat, C

Pons, M

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Univ Barcelona, Dept Quim Organ, E-08028 Barcelona, Spain

[3] Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Unitat Biofis, E-08193 Barcelona, Spain

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 03期

关键词：

D O I：

10.1021/ja0170192

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Addition of Tb3+ to purple membrane (PM) suspensions changes the orientation of the menbrane normal from parallel to perpendicular with respect to the magnetic field. Residual dipolar couplings measured in protein L in the presence of PM are scaled by a factor of -1/2. NMR line broadening and cross-correlation effects induced by the addition of PM are partially reversed by Tb3+ but not by Tm3+ that has no effect on the orientation of PM. This is interpreted as the result of anisotropically restricted motion of protein L in the transiently PM-bound form. Copyright © 2002 American Chemical Society.

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页码：374 / 375

页数：2

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