Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme

被引：231

作者：

Iwai, H

Züger, S

Jin, J

Tam, PH

机构：

[1] Dept Chem, Saskatoon, SK S7N 5C9, Canada

[2] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5C9, Canada

[3] Univ Helsinki, Inst Biotechnol, Res Program Struct Biol & Biophys, FIN-00014 Helsinki, Finland

来源：

FEBS LETTERS | 2006年 / 580卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

protein splicing; intein; segmental isotopic labelling; NMR;

D O I：

10.1016/j.febslet.2006.02.045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE possesses robust trans-splicing activity with an efficiency of > 98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 (Ssp DnaE). Both the N- and C-terminal parts of the split Npu DnaE intein can be substituted with the corresponding fragment of Ssp DnaE without loss of trans-splicing activity. Protein splicing with the Npu DnaE(N) is also more tolerant of amino acid substitutions in the C-terminal extein sequence. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：1853 / 1858

页数：6

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