Aquaporin 1 expression in human temporomandibular disc

被引：21

作者：

Loreto, Carla ^{[1
]}

Lo Castro, Ester ^{[2
]}

Musumeci, Giuseppe ^{[1
]}

Loreto, Federico ^{[3
]}

Rapisarda, Giuseppe ^{[4
]}

Rezzani, Rita ^{[5
]}

Castorina, Sergio ^{[1
]}

Leonardi, Rosalia ^{[2
]}

Rusu, Mugurel Constantin ^{[6
]}

机构：

[1] Univ Catania, Dept Biomed Sci, Anat Sect, I-95131 Catania, Italy

[2] Univ Catania, Dent Unit 2, Dept Med & Surg Sci, I-95131 Catania, Italy

[3] Descartes Univ, Sch Med, Paris, France

[4] Dept Vet Publ Hlth, Gen Pathol & Pathol Anat Sect, Catania, Italy

[5] Univ Brescia, Dept Biomed Sci & Biotechnol, Brescia, Italy

[6] Carol Davila Univ Med & Pharm, Discipline Anat, Bucharest, Romania

来源：

ACTA HISTOCHEMICA | 2012年 / 114卷 / 07期

关键词：

Aquaporin; 1; Temporomandibular joint disc; Immunohistochemistry; Western blotting; Human; WATER CHANNELS; JOINT DISC; ARTICULAR-CARTILAGE; PROTEOGLYCAN SYNTHESIS; MEMBRANE TRANSPORTERS; INTERVERTEBRAL DISC; MOLECULAR-BIOLOGY; GENE-EXPRESSION; CELL-VOLUME; CHONDROCYTES;

D O I：

10.1016/j.acthis.2012.01.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Aquaporins (AQPs) are a family of hydrophobic membrane channel proteins. The expression of several AQP isoforms has been investigated in different human tissues, including the orofacial region. However, information on the role and localization of AQP1 in joints is limited, and no data are available on aquaporins in the normal temporomandibular joint (TMJ) disc. Sixteen human TMJ discs without degenerative changes were taken from fresh cadavers to investigate the presence and distribution of AQP1 by immunohistochemistry. The aim of the study was to gain additional insights into the biomolecular composition of aquaporins and their role in homeostasis of the TMJ. Porcine TMJ discs were also studied by Western blotting for comparison. Scattered AQP1 immunoexpression was detected in human disc cells, documenting its constitutive expression, but differences amongst the three disc regions were not significant. AQP1 expression was demonstrated in porcine TMJ disc by Western blotting. Our findings suggest that AQP1 is normally expressed in the TMJ disc and confirm a role for it in the maintenance of TMJ homeostasis. Further studies are needed to elucidate expression patterns of aquaporins in diseased TMJ discs. (c) 2012 Elsevier GmbH. All rights reserved.

引用

页码：744 / 748

页数：5

共 39 条

[1]

Age changes in the cells of the intra-articular disc of the temporomandibular joints of rats and marmosets [J].