Ryanodine receptor type III (Ry(3)R) identification in mouse parotid acini - Properties and modulation of [H-3]ryanodine-binding sites

Immunoblot analysis and [H-3]ryanodine binding were used to characterize and identify ryanodine receptors (RyRs) in nonexcitable mouse parotid acini. Western analysis revealed ryanodine receptor type III (Ry(3)R) to be the only detectable isoform in parotid microsomal membranes. Binding of [H-3]ryanodine to microsomal fractions was dependent on Ca2+, salt, pH, and temperature. At 23 degrees C, and in the presence of 0.5 M KCl and 100 mu M Ca2+, [H-3]ryanodine bound specifically to membranes with high affinity (K-d = 6 nM); maximum binding capacity (B-max) was 275 fmol/mg protein. Mg2+ and ruthenium red inhibited [H-3]ryanodine binding: (IC50 = 1.4 mM and 0.5 mu M, respectively), 4-Chloro-3-ethylphenol enhanced the binding of [H-3]ryanodine 2.5-fold; whereas ATP and caffeine were much less efficacious toward activating Ry(3)R (56% and 18% maximal enhancement, respectively), Bastadin, a novel modulator of the 12-kDa FK506 binding protein RyR complex, increased [H-3]ryanodine binding 3-4-fold by enhancing K-d. The immunosuppressant FK506 enhanced [H-3]ryanodine receptor occupancy at >100 mu M and antagonized the action of bastadin, suggesting that an immunophilin modulates Ry(3)R in parotid acini, These results suggest that Ry(3)R may play an important role in Ca2+ homeostasis in mouse parotid acini.