Isoprenoid biosynthesis via the methylerythritol phosphate pathway.: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate:: chemical synthesis and formation from methylerythritol cyclodiphosphate by a cell-free system from Escherichia coli

被引：70

作者：

Wolff, M

Seemann, M

Grosdemange-Billiard, C

Tritsch, D

Campos, N

Rodríguez-Concepción, M

Boronat, A

Rohmer, M

机构：

[1] Univ Strasbourg, CNRS, Inst Le Bel, F-67070 Strasbourg, France

[2] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

来源：

TETRAHEDRON LETTERS | 2002年 / 43卷 / 14期

关键词：

biosynthesis; gcpE; 2-C-methyl-D-erythritol; 4-phosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; isoprenoids; lytB;

D O I：

10.1016/S0040-4039(02)00293-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

2-C-Methyl-D-erythritol cyclodiphosphate is converted into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by a cell-free system from an Escherichia coli strain overexpressing the gcpE gene. The latter diphosphate, representing probably the last intermediate in the MEP pathway for isoprenoid biosynthesis, was identified by comparison with reference material obtained by chemical synthesis. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：2555 / 2559

页数：5

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