Effect of dietary docosahexaenoic acid on desaturation and uptake in vivo of isotope-labeled oleic, linoleic, and linolenic acids by male subjects

被引:85
作者
Emken, EA
Adlof, RO
Duval, SM
Nelson, GJ
机构
[1] Midw Res Consultants, Princeville, IL 61559 USA
[2] ARS, USDA, NCAUR, Peoria, IL 61604 USA
[3] Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA
关键词
D O I
10.1007/s11745-999-0424-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of dietary docosahexaenoic acid (22:6n-3, DHA) on the metabolism of oleic, linoleic, and linolenic acids was investigated in male subjects (n = 6) confined to a metabolic unit and fed diets containing 6.5 or <0.1 g/d of DHA for 90 d. At the end of the diet period, the subjects were fed a mixture of deuterated triglycerides containing 18:1n-9[d6], 18:2n-6[d2], and 18:3n-3[d4]. Blood samples were drawn at 0, 2, 4, 6, 8, 12, 24, 48, and 72 h. Methyl esters of plasma total lipids, triglycerides, phospholipids, and cholesterol esters were analyzed by gas chromatography-mass spectrometry. Chylomicron triglyceride results show that the deuterated fatty acids were equally well absorbed and diet did not influence absorption. Compared to the low-DHA diet (LO-DHA), clearance of the labeled fatty acids from chylomicron triglycerides was modestly higher for subjects fed the high DHA diet (HI-DHA). DHA supplementation significantly reduced the concentrations of most n-6[d2] and n-3[d4] long-chain fatty acid (LCFA) metabolites in plasma lipids, Accumulation of 20:5n-3[d4] and 22:6n-3[d4] was depressed by 76 and 88%, respectively. Accumulations of 20:3n-6[d2] and 20:4n-6[d2] were both decreased by 72%. No effect of diet was observed on acyltransferase selectivity or on uptake and clearance of 18:1n-9[d6], 18:2n-6[d2], and 18:3n-3[d4]. The results indicate that accumulation of n-3 LCFA metabolites synthesized from 18:3n-3 in typical U.S. diets would be reduced from about 120 to 30 mg/d by supplementation with 6.5. g/d of DHA. Accumulation of n-6 LCFA metabolites synthesized from 18:2n-6 in U.S. diets is estimated to be reduced from about 800 to 180 mg/d. This decrease is two to th ree times the amount of n-6 LCFA in a typical U.S. diet. These results support the hypothesis that health benefits associated with DHA supplementation are the combined result of reduced accretion of n-6 LCFA metabolites and an increase in n-3 LCFA levels in tissue lipids.
引用
收藏
页码:785 / 791
页数:7
相关论文
共 30 条
[1]   SYNTHESIS OF METHYL CIS-9-OCTADECENOATE-14,14,15,15,17,18-D6 [J].
ADLOF, RO ;
EMKEN, EA .
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1978, 15 :97-104
[3]   LARGE-SCALE PREPARATION OF LINOLEIC ACID-D2-ENRICHED TRIGLYCERIDES FROM CREPIS-ALPINA SEED OIL [J].
ADLOF, RO ;
EMKEN, EA .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1993, 70 (08) :817-819
[4]  
Agren JJ, 1996, EUR J CLIN NUTR, V50, P765
[5]   DESATURATION AND CHAIN ELONGATION OF N-3 AND N-6 POLYUNSATURATED FATTY-ACIDS IN THE HUMAN CACO-2 CELL-LINE [J].
CHEN, Q ;
NILSSON, A .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1166 (2-3) :193-201
[6]  
Christie WW, 1973, LIPID ANAL, P85
[7]  
*CONS FOOD EC I, 1992, USDA AGR HDB, V8
[8]   Effect of dietary arachidonic acid on metabolism of deuterated linoleic acid by adult male subjects [J].
Emken, EA ;
Adlof, RO ;
Duval, SM ;
Nelson, GJ .
LIPIDS, 1998, 33 (05) :471-480
[9]   DIETARY LINOLEIC-ACID INFLUENCES DESATURATION AND ACYLATION OF DEUTERIUM-LABELED LINOLEIC AND LINOLENIC ACIDS IN YOUNG-ADULT MALES [J].
EMKEN, EA ;
ADLOF, RO ;
GULLEY, RM .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1213 (03) :277-288
[10]   Influence of dietary arachidonic acid on metabolism in vivo of 8cis,11cis,14-eicosatrienoic acid in humans [J].
Emken, EA ;
Adlof, RO ;
Duval, SM ;
Nelson, GJ .
LIPIDS, 1997, 32 (04) :441-448