Tyrosinase inhibition organic phase biosensor for triazinic and benzotriazinic pesticide analysis (part two)

被引：20

作者：

Campanella, L ^{[1
]}

Dragone, R ^{[1
]}

Lelo, D ^{[1
]}

Martini, E ^{[1
]}

Tomassetti, M ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 384卷 / 04期

关键词：

triazine; benzotriazine; pesticide analysis; OPEE; tyrosinase inhibition;

D O I：

10.1007/s00216-005-0175-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Several triazine pesticides, such as atrazine, are much more soluble in several organic solvents, such as chloroform, than in water. Our recent research was aimed at analyzing this class of pesticides using tyrosinase OPEE (organic phase enzyme electrodes), exploiting their inhibiting action on the tyrosinase enzyme when operating in water-saturated chloroform medium. In this work we studied the response of a tyrosinase inhibition enzyme sensor to several triazinic (simazine, propazine, terbuthylazine) and benzotriazinic (azinphos-ethyl and azinphos-methyl) pesticides (LOD=0.5x10(-9) mol l(-1)). Recovery trials were also performed in vegetal matrixes (corn, barley, lentils). Lastly, the effect of the solvent (chloroform or water) on the inhibition process was investigated via Hill's equation and the diffusion of analyte from the solvent to the enzyme membrane.

引用

页码：915 / 921

页数：7

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