Novel G-protein complex whose requirement is linked to the translational status of the cell

被引:56
作者
Carr-Schmid, A
Pfund, C
Craig, EA
Kinzy, TG
机构
[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA
[2] Ctr Canc, Piscataway, NJ 08854 USA
[3] Univ Wisconsin, Dept Biomol Chem, Madison, WI 53706 USA
关键词
D O I
10.1128/MCB.22.8.2564-2574.2002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
G proteins, which bind and hydrolyze GTP, are involved in regulating a variety of critical cellular processes, including the process of protein synthesis. Many members of the subfamily of elongation factor class G proteins interact with the ribosome and function to regulate discrete steps during the process of protein synthesis. Despite sequence similarity to factors involved in translation, a role for the yeast Hbs1 protein has not been defined. In this work we have identified a genetic relationship between genes encoding components of the translational apparatus and HBS1. HBS1, while not essential for viability, is important for efficient growth and protein synthesis under conditions of limiting translation initiation. The identification of an Hbs1p-interacting factor, Dom34p, which shares a similar genetic relationship with components of the translational apparatus, suggests that Hbs1p and Dom34p may function as part of a complex that facilitates gene expression. Dom34p contains an RNA binding motif present in several ribosomal proteins and factors that regulate translation of specific mRNAs. Thus, Hbs1p and Dom34p may function together to help directly or indirectly facilitate the expression either of specific mRNAs or under certain cellular conditions.
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页码:2564 / 2574
页数:11
相关论文
共 55 条
[1]   2 GENES FOR RIBOSOMAL PROTEIN-51 OF SACCHAROMYCES-CEREVISIAE COMPLEMENT AND CONTRIBUTE TO THE RIBOSOMES [J].
ABOVICH, N ;
ROSBASH, M .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (09) :1871-1879
[2]   EFFECT OF RP51 GENE DOSAGE ALTERATIONS ON RIBOSOME SYNTHESIS IN SACCHAROMYCES-CEREVISIAE [J].
ABOVICH, N ;
GRITZ, L ;
TUNG, L ;
ROSBASH, M .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3429-3435
[3]   Ski7p G protein interacts with the exosome and the Ski complex for 3′-to-5′ mRNA decay in yeast [J].
Araki, Y ;
Takahashi, S ;
Kobayashi, T ;
Kajiho, H ;
Hoshino, S ;
Katada, T .
EMBO JOURNAL, 2001, 20 (17) :4684-4693
[4]   A MUTATION ALLOWING AN MESSENGER-RNA SECONDARY STRUCTURE DIMINISHES TRANSLATION OF SACCHAROMYCES-CEREVISIAE ISO-1-CYTOCHROME-C [J].
BAIM, SB ;
PIETRAS, DF ;
EUSTICE, DC ;
SHERMAN, F .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) :1839-1846
[5]  
BANIAHMAD C, 1994, BIOTECHNIQUES, V16, P194
[6]   USE OF A SCREEN FOR SYNTHETIC LETHAL AND MULTICOPY SUPPRESSEE MUTANTS TO IDENTIFY 2 NEW GENES INVOLVED IN MORPHOGENESIS IN SACCHAROMYCES-CEREVISIAE [J].
BENDER, A ;
PRINGLE, JR .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (03) :1295-1305
[7]  
BOEKE JD, 1987, METHOD ENZYMOL, V154, P164
[8]   The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p [J].
Bonnerot, C ;
Boeck, R ;
Lapeyre, B .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (16) :5939-5946
[9]  
BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
[10]  
BOURNE HR, 1990, NATURE, V348, P25