Induction of nodavirus disease in seabass, Dicentrarchus labrax, using different infection models

被引:38
作者
Skliris, GP [1 ]
Richards, RH [1 ]
机构
[1] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
关键词
challenge; cohabitation; Dicentrarchus labrax; histopathology; immune response; nodavirus; virology;
D O I
10.1016/S0168-1702(99)00061-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim of this study was to investigate the susceptibility of juvenile and adult seabass, which are generally thought to be refractory to nodavirus. Moreover, preliminary immunological studies were performed to examine the immune response of adult seabass. Successful transmission of the disease was experimentally demonstrated in juvenile and adult seabass as ascertained by the presence of the clinical signs of the disease, re-isolation of the virus in the SSN-1 cell line and subsequent confirmation by histopathology and immunohistochemistry. Bigger seabass not only developed the clinical disease but also suffered mortalities. Serum neutralisation titres were considered low in this study. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:85 / 93
页数:9
相关论文
共 26 条
[1]   PATHOGENICITY OF THE CAUSATIVE AGENT OF VIRAL NERVOUS NECROSIS DISEASE IN STRIPED JACK, PSEUDOCARANX-DENTEX (BLOCH AND SCHNEIDER) [J].
ARIMOTO, M ;
MORI, K ;
NAKAI, T ;
MUROGA, K ;
FURUSAWA, I .
JOURNAL OF FISH DISEASES, 1993, 16 (05) :461-469
[2]  
BELLANCE R, 1988, CARAIBES MED, P105
[3]   ENCEPHALOMYELITIS AMONG TURBOT ASSOCIATED WITH A PICORNAVIRUS-LIKE AGENT [J].
BLOCH, B ;
GRAVNINGEN, K ;
LARSEN, JL .
DISEASES OF AQUATIC ORGANISMS, 1991, 10 (01) :65-70
[4]   Picorna-like virus associated with mortality and a spongious encephalopathy in grouper Epinephelus malabaricus [J].
Boonyaratpalin, S ;
Supamattaya, K ;
Kasornchandra, J ;
Hoffmann, RW .
DISEASES OF AQUATIC ORGANISMS, 1996, 26 (01) :75-80
[5]  
BOVO G, 1996, B SOC ITAL PATOL ITT, V8, P52
[6]   VIRAL-INFECTION (PICORNA-LIKE VIRUS) ASSOCIATED WITH MASS MORTALITIES IN HATCHERY-REARED SEA-BASS (DICENTRARCHUS-LABRAX) LARVAE AND JUVENILES [J].
BREUIL, G ;
BONAMI, JR ;
PEPIN, JF ;
PICHOT, Y .
AQUACULTURE, 1991, 97 (2-3) :109-116
[7]   PURIFICATION AND CHARACTERIZATION OF 2 FISH ENCEPHALITIS VIRUSES (FEV) INFECTING LATES-CALCARIFER AND DICENTRARCHUS-LABRAX [J].
COMPS, M ;
PEPIN, JF ;
BONAMI, JR .
AQUACULTURE, 1994, 123 (1-2) :1-10
[8]  
Dixon P, 1997, DEV BIOLOGICALS, V90, P221
[9]   Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax [J].
Frerichs, GN ;
Rodger, HD ;
Peric, Z .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2067-2071
[10]   Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis [J].
Fukuda, Y ;
Nguyen, HD ;
Furuhashi, M ;
Nakai, T .
FISH PATHOLOGY, 1996, 31 (03) :165-170