All-trans retinoic acid increases peptidylarginine deiminases in a newborn rat keratinocyte cell line

被引:12
作者
Ishigami, A [1 ]
Ohsawa, T [1 ]
Watanabe, K [1 ]
Senshu, T [1 ]
机构
[1] TOKYO METROPOLITAN INST GERONTOL, DEPT EXPTL BIOL, ITABASHI KU, TOKYO 173, JAPAN
关键词
D O I
10.1006/bbrc.1996.0888
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied the expression of peptidylarginine deiminases (EC 3.5.3.15) in an immortalized newborn rat keratinocyte cell line. No measurable enzyme activities were noted in either growing or confluent cultures. The enzyme activity was increased by all-trans retinoic acid in dose- and time-dependent manners. The enzyme activity was resolved into two peaks by anion exchange chromatography. The minor peak resembled enzyme preparations obtained from the epidermis in earlier studies. The major peak was indistinguishable from rat muscle peptidylarginine deiminase in the chromatographic and Western blotting profiles. Northern blot hybridization showed a major band in retinoic acid-treated cells migrating slightly behind muscle peptidylarginine deiminase mRNA. (C) 1996 Academic Press, Inc.
引用
收藏
页码:299 / 303
页数:5
相关论文
共 22 条
[1]   FILAGGRIN PRODUCTION BY CULTURED HUMAN EPIDERMAL-KERATINOCYTES AND ITS REGULATION BY RETINOIC ACID [J].
ASSELINEAU, D ;
DALE, BA ;
BERNARD, BA .
DIFFERENTIATION, 1990, 45 (03) :221-229
[2]   THE GROWTH AND DIFFERENTIATION OF CULTURED NEWBORN RAT KERATINOCYTES [J].
BADEN, HP ;
KUBILUS, J .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1983, 80 (02) :124-130
[3]   DIFFERENTIAL MODULATION OF EPIDERMAL KERATINIZATION IN IMMORTALIZED (HACAT) AND TUMORIGENIC HUMAN SKIN KERATINOCYTES (HACAT-RAS) BY RETINOIC ACID AND EXTRACELLULAR CA2+ [J].
BREITKREUTZ, D ;
STARK, HJ ;
PLEIN, P ;
BAUR, M ;
FUSENIG, NE .
DIFFERENTIATION, 1993, 54 (03) :201-217
[4]   RETINOIC ACID SUPPRESSION OF LORICRIN EXPRESSION IN RECONSTITUTED HUMAN SKIN CULTURED AT THE LIQUID-AIR INTERFACE [J].
BROWN, LJ ;
GEESIN, JC ;
ROTHNAGEL, JA ;
ROOP, DR ;
GORDON, JS .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (06) :886-890
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]  
FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266
[7]   PROPERTIES OF PEPTIDYLARGININE DEIMINASE FROM THE EPIDERMIS OF NEWBORN RATS [J].
FUJISAKI, M ;
SUGAWARA, K .
JOURNAL OF BIOCHEMISTRY, 1981, 89 (01) :257-263
[8]   ANTISENSE PROFILAGGRIN RNA DELAYS AND DECREASES PROFILAGGRIN EXPRESSION AND ALTERS IN-VITRO DIFFERENTIATION OF RAT EPIDERMAL-KERATINOCYTES [J].
HAYDOCK, PV ;
BLOMQUIST, C ;
BRUMBAUGH, S ;
DALE, BA ;
HOLBROOK, KA ;
FLECKMAN, P .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 101 (02) :118-126
[9]   PURIFICATION AND PROPERTIES OF A BRAIN ENZYME WHICH DEIMINATES PROTEINS [J].
KUBILUS, J ;
BADEN, HP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 745 (03) :285-291
[10]   PARTIAL-PURIFICATION AND SPECIFICITY OF AN ARGININE-CONVERTING ENZYME FROM BOVINE EPIDERMIS [J].
KUBILUS, J ;
WAITKUS, RF ;
BADEN, HP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 615 (01) :246-251