Diversity associated with the second expressed HLA-DRB locus in the human population

Although diversity within the HLA-DRB region is predominantly focused in the DRB1 gene, the second expressed DRB loci, DRB3, DRB4, and DRB5, also exhibit variation. Within DRB1*15 or DRB1*16 haplotypes, four new variants were identified: 1) two new DRB5 alleles, DRB5*0104 and DRB5*0204, 2) a haplotype carrying a DRB1*15 or *16 allele without the usual accompanying DRB5 allele, and 3) a haplotype carrying a DRB5*0101 allele without a DRB1*15 or *16 allele. The evolutionary origins of these haplotypes were postulated based on their associations with the DRB6 pseudogene. Within HLA haplotypes which carry DRB3, a new DRB3*0205 allele and one unusual DRB3 association were identified. Finally, two new null DRB4 alleles are described: DRB4*0201N, which exhibits a deletion in the second exon, and a second allele, DRB4*null, which lacks the second exon completely. Gene conversion-like events and variation in the number of functional genes through reciprocal recombination and inactivation contribute to the diversity observed in the second expressed HLA-DRB loci.