The influence of organic solvents on phospholipid transformations by phospholipase D in emulsion systems

被引：39

作者：

Hirche, F

Koch, MHJ

Konig, S

Wadewitz, T

UlbrichHofmann, R

机构：

[1] UNIV HALLE WITTENBERG, DEPT BIOCHEM & BIOTECHNOL, D-06099 HALLE, GERMANY

[2] UNIV HALLE WITTENBERG, DEPT CHEM, D-06099 HALLE, GERMANY

[3] DESY, EUROPEAN MOL BIOL LAB, D-2000 HAMBURG, GERMANY

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1997年 / 20卷 / 06期

关键词：

interfacial tension; phospholipase D; phospholipid transformation; solvent; X-ray scattering; ENZYMATIC-SYNTHESIS; PHOSPHATIDYLGLYCEROL SYNTHESIS; SYNCHROTRON RADIATION; INTERFACIAL AREA; PHOSPHATIDYLCHOLINE; CONVERSION; TRANSPHOSPHATIDYLATION; PHOSPHATIDYLSERINE; SCATTERING; EQUILIBRIA;

D O I：

10.1016/S0141-0229(97)00172-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phospholipase D is an accepted tool in the transformation of phospholipids which is preferably performed in aqueous-organic emulsion systems. The hydrolysis of phosphatidylcholine and its transphosphatidylation with 1-butanol are extremely different in hexane and diethyl ether. Small amounts of primary ol secondary alcohols (<2%) are able to mediate the otherwise very slow reaction in hexane/buffer. The reaction systems were characterized by X-ray scattering, water content in the organic phases, and interfacial areas and tensions. The different packing density of the amphiphilic phosphatidylcholine molecules at the interface where the enzymatic reaction mainly takes place appears to be one of the major causes for the large solvent effects. (C) 1997 by Elsevier Science Inc.

引用

页码：453 / 461

页数：9

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